中国康复理论与实践2016，Vol.22Issue(11)：1252-1258,7.DOI:10.3969/j.issn.1006-9771.2016.11.003
MicroRNA-9调控室管膜下区干细胞增殖在电针治疗局灶性脑缺血中的作用
Effect of MicroRNA-9 Regulating Stem Cell Proliferation in Subventricular Zone on Electroacupuncture for Fo-cal Cerebral Ischemia
摘要
Abstract
Objective To investigate the effect of electroacupuncture (EA) at Quchi (LI11) and Zusanli (ST36) acupoints on rats with fo-cal cerebral ischemia, and whether it was mediated by microRNA-9 (miR-9) regulating neural stem cells proliferation in subventricular zone. Methods Fifty-two Sprague-Dawley rats were randomly assigned into sham group (n=12), model group (n=12), EA group (n=12), EA+dimethylsulfoxide (DMSO) group (n=8) and EA+miR-9 mimics group (n=8). The middle cerebral artery occlusion model was induced. DMSO and miR-9 mimics were performed by intracerebroventricular injection 30 minutes before modeling. The EA group, EA+ DMSO group and EA+miR-9 mimics group were electroacupunctured at Quchi and Zusanli acupoints the next day after modeling. All the groups were intraperitoneally injected with bromodeoxyuridine (BrdU). The BrdU co-localized with Nestin was observed by immunofluorescence. The expression of miR-9 in subventricular zone was detected by real time qPCR. Results The neurological deficit score was lower (t=9.600, P=0.006), and the infarct volume was smaller (t=14.080, P=0.024) in the electroacupuncture group than in the model group. The expression of Nestin and BrdU co-localizated with Nestin increased;while the expression of miR-9 decreased in subventricular zone (P<0.05) in the electoacupuncture group. However, the expression of Nestin, BrdU and BrdU co-localizated with Nestin was lower in the EA+miR-9 mim-ics group than in the EA+DMSO group. Conclusion Electroacupuncture at Quchi and Zusanli acupoints may promote the neural stem cells proliferation in subventricular zone by downregulating the miR-9 expression.关键词
局灶性脑缺血/电针/神经干细胞/microRNA-9/大鼠Key words
focal cerebral ischemia/electroacupuncture/neural stem cells/microRNA-9/rats分类
医药卫生引用本文复制引用
黄佳,郑薏,姚建宁,叶晓倩,王露露,陶静,柳维林..MicroRNA-9调控室管膜下区干细胞增殖在电针治疗局灶性脑缺血中的作用[J].中国康复理论与实践,2016,22(11):1252-1258,7.基金项目
1.福建省卫生厅青年科研课题(No.2014-1-70)；2.福建省自然科学基金(No.2016J01382)。 （No.2014-1-70）
