中国康复理论与实践2016，Vol.22Issue(11)：1259-1263,5.DOI:10.3969/j.issn.1006-9771.2016.11.004
电针对失神经骨骼肌萎缩大鼠胰岛素样生长因子1、肌肉生长抑制素及肌卫星细胞增殖的影响
Effects of Electroacupuncture on Insulin-like Growth Factor-1, Myostatin and Satellite-cell Proliferation in Rats with Denervated Skeletal Muscle Atrophy
摘要
Abstract
Objective To observe effects and mechanism of electroacupuncture (EA) on denervated skeletal muscle atrophy. Methods Forty-nine male Sprague-Dawley rats were randomly divided into normal group (group A, n=7), natural recovery group (group B, n=21) and EA group (group C, n=21). The groups B and C, established the model of denervated skeletal muscle atrophy by transecting the sciatic nerve of rats, were divided into subgroups of 7 days, 14 days, 21 days postoperation, seven in each subgroup. Electroacupuncture was given to the group C at Zusanli (ST36) and Chengshan (BL57) once a day since 24 hours after modeling. The muscle wet weight ratio of the affected gas-trocnemius was determined. Cross-sectional area and fiber diameter of the gastrocnemius were measured with HE staining. The expression of insulin-like growth factor-1 (IGF-1), Myostatin and proliferating cell nuclear antigen (PCNA) protein and gene in the gastrocnemius were detected with Western blotting and RT-PCR. Results The wet weight ratio, cross-sectional area and fiber diameter were less in the groups B and C than in the group A (P<0.001), and they were more in the group C than in the group B (P<0.001). Compared with the group B, the pro-tein and gene of IGF-1, PCNA increased in the group C (P<0.05), while the Myostatin decreased (P<0.05). Conclusion Electroacupuncture can increase the expression of IGF-1 and decrease the expression of Myostatin, to promote the proliferation of satellite cell, which may re-late with the prevention of denervated skeletal muscle atrophy.关键词
失神经/骨骼肌萎缩/电针/胰岛素样生长因子1/肌肉生长抑制素/增殖细胞核抗原/肌卫星细胞/大鼠Key words
denervation/muscle atrophy/electroacupuncture/insulin-like growth factor-1/Myostatin/proliferating cell nuclear anti-gen/satellite cell/rats分类
医药卫生引用本文复制引用
高睿琦,唐成林,曹净,郭全虎,张毅,田源,袁海洲..电针对失神经骨骼肌萎缩大鼠胰岛素样生长因子1、肌肉生长抑制素及肌卫星细胞增殖的影响[J].中国康复理论与实践,2016,22(11):1259-1263,5.基金项目
1.国家自然科学基金项目(No.81273870)；2.重庆市卫计委中医药科研项目(No.ZY20150249)。 （No.81273870）
