中国康复理论与实践2016，Vol.22Issue(11)：1264-1268,5.DOI:10.3969/j.issn.1006-9771.2016.11.005
电针委中穴对大鼠退变腰椎间盘组织中Bcl-xL、Bax表达的影响
Effects of Electroacupuncture at Weizhong on Expression of Bcl-xL and Bax in Rats with Lumbar Disc Degenera-tion
摘要
Abstract
Objective To explore the effect of electroacupuncture at Weizhong (BL40) acupoint on the expression of Bcl-xL and Bax in rats with lumbar disc degeneration. Methods Thirty male adult Sprague-Dawley rats were randomly divided into sham-operated group (n=10), model group (n=10), and electroacupuncture group (n=10). The lumbar disc degeneration model was established by puncturing the an-nulus fibrosis in the model group and electroacupuncture group. One month after modeling, the electroacupuncture group was electroacu-punctured at Weizhong acupoint for four weeks. HE staining was used to observe the pathological changes. Immunohistochemical staining was used to observe the expression of Bax in the lumbar disc tissue. Western blotting was used to detect the expression of Bcl-xL and Bax protein. Results HE staining showed that the degrees of lumbar disc degeneration from light to severe was ranged as: the sham-operated group, the electroacupuncture group and the model group. The expression of Bcl-xL was lower (P<0.05), and the expression of Bax was higher (P<0.01) in the model group than in the sham-operated group, which was opposite (P<0.01) in the electroacupuncture group than in the model group. Conclusion Electroacuponcture at Weizhong may upregulate the expression of Bcl-xL and downregulate the expression of Bax in rats with lumbar disc degeneration.关键词
腰椎间盘退变/电针/委中穴/凋亡因子/大鼠Key words
lumbar disc degeneration/electroacupuncture/Weizhong/apoptosis factor/rats分类
医药卫生引用本文复制引用
何挺超,陈少清,林建平,刘午龙,夏梦,王诗忠..电针委中穴对大鼠退变腰椎间盘组织中Bcl-xL、Bax表达的影响[J].中国康复理论与实践,2016,22(11):1264-1268,5.基金项目
1.国家自然科学基金项目(No.81303017)；2.福建省卫生厅医学创新项目(No.2014-CXB-20)。 （No.81303017）
