中国康复理论与实践2016，Vol.22Issue(11)：1269-1275,7.DOI:10.3969/j.issn.1006-9771.2016.11.006
黄芪多糖对大鼠脊髓损伤后运动功能和脊髓病理的效果
Effect of Astragalus Polysaccharide on Motor Function and Pathology after Spinal Cord Injury in Rats
摘要
Abstract
Objective To observe the effect of astragalus polysaccharide on motor function and pathology after spinal cord injury in rats. Methods Seventy adult Sprague-Dawley rats were selected as normal group (n=10), injury group (n=30) and treatment group (n=30), and the injury group and the treatment group were divided into 7 days, 14 days, 28 days subgroups. The injury group and the treatment group were modeled with Allen's mode at T10 (10 g×25 mm). The treatment group was injected with astragalus polysaccharide 10 mg/kg per day af-ter injury. They were rated with Basso-Beattie-Bresnahan (BBB) scores 7 days, 14 days and 28 days after injury, while the morphology was observed with HE staining, Nylon body was observed with Nissl staining, and myelin sheath was observed with eriochrome cyanine stain-ing. Results The BBB score was significantly higher in the treatment group than in the injury group at each time point (P<0.05). There was a large number of necrotic tissue in the injured cords and cystic cavity began to form 7 and 14 days after injury. Cystic cavity formed basical-ly and surrounded with dense scar 28 days after injury. The necrosis and cystic cavity alleviated in the treatment group at each time point. Demyelination and myelin sheaths loose were found 7 days after injury, and aggravated with the time. There was a cystic cavity in trauma center 28 days after injury. The demyelination and myelin sheaths loose relieved at each time point in the treatment group. Nissl bodies be-gan to coalesce 7 days after injury, and aggravated 14 days after injury, coalesced completely 28 days after injury. Nissl body coalesced alle-viatively in the treatment group at each time point. Conclusion Astragalus polysaccharide may reduce the damage and promote the recovery of motor function after spinal cord injury in rats.关键词
脊髓损伤/黄芪多糖/运动功能/病理/大鼠Key words
spinal cord injury/astragalus polysaccharide/motor function/pathology/rats分类
医药卫生引用本文复制引用
郑利强,伍亚民,石永江,江琼..黄芪多糖对大鼠脊髓损伤后运动功能和脊髓病理的效果[J].中国康复理论与实践,2016,22(11):1269-1275,7.基金项目
国家自然科学基金项目(No.30772299)。 （No.30772299）
