中国水稻科学2016，Vol.30Issue(6)：567-576,10.DOI:10.16819/j.1001-7216.2016.6017
水稻根系中磷高效吸收和利用相关基因表达对低磷胁迫的应答
Response of Gene Expression Related to Efficient Phosphorus Absorption and Utilization to Low-P Stress in Rice Roots
摘要
Abstract
It is important to elucidate the mechanism of low phosphorus tolerance in rice for the improvement of utilization efficiency of soil phosphorus.With two different rice varieties,low-P tolerant variety Yi 2434 and low-P sensitive variety Tongjing 981 ,the gene expression related to phosphorus absorption and utilization in rice seedling roots were determined by using gene chip and qRT-PCR technology.The phosphorus content showed that compared with the control group,the phosphorus content in the leaves of Yi 2434 decreased by a less percentage than that in Tongjing 981 under low-P stress,indicating that Yi 2434 had a high efficiency in phosphorus absorption from low phosphorus medium compared with Tongjing 981 .The gene chip results showed that the expressions of P HR1 , osa-miR399 s and SP Xs triggered the phosphorus starvation signal pathway,and the induced gene expressions of APA ,PAPs ,MPE ,PA ,PEPC and VDAC1 ,C4-DT/MAT enhanced the synthesis and secretion of organophos-phorus hydrolase and organic acid,promoted the reactivation of insoluble phosphorus in media,and the induced gene expressions of OsPT2 and OsPT6 accelerated the absorption of phosphorus in Yi 2434 roots under low-P stress. qRT-PCR results showed that the transcriptional levels of the eight key genes (P HR1 ,SP X ,PAP ,APA ,PEPC , MFS ,OsPT 2,OsPT6 )related to phosphorus starvation signal transduction,phosphorus activation and efficient absorption were continuously increased along with the low-P treatment in Yi 2434 roots.Furthermore, the transcriptional levels of these eight genes in Yi 2434 roots were significantly higher than those in Tongjing 981 roots under the low-P stress and the induced expression of P HR1 ,APA ,OsPT2 genes in Tongjing 981 roots was very low under low-P stress.Additionally,the activities of both acid phosphatase (APA)in the tissues of roots and secreted by roots in Yi 2434 increased more significantly than that in Tongjing 981 ,which is possibly one of the key pathway to confer Yi 2434 higher tolerance to the low-P stress than Tongjing 981 .关键词
水稻/低磷胁迫/基因表达/磷活化/磷高效吸收Key words
rice/low phosphorus stress/gene expression/phosphorus activation/efficient phosphorus absorption分类
农业科技引用本文复制引用
胡训霞,史春阳,丁艳,张萍,葛永胜,刘玉金,王泽港,葛才林..水稻根系中磷高效吸收和利用相关基因表达对低磷胁迫的应答[J].中国水稻科学,2016,30(6):567-576,10.基金项目
农业公益性行业科研专项(201103007) （201103007）
