中国水稻科学2016，Vol.30Issue(6)：587-592,6.DOI:10.16819/j.1001-7216.2016.6056
一个水稻小热休克蛋白基因的克隆和鉴定
Cloning and Characterization of a Small Heat Shock Protein (SHSP)Gene in Rice Plant
摘要
Abstract
Small heat shock proteins (SHSPs)exist widely in all kinds of creatures and play an important role in both development and stress responses.A gene encoding a SHSP (OsSHSP1 7.6 )was cloned from Oryza sativa and the sequence assembly showed the encoding region of OsSHSP1 7.6 was 477 bp in length,encoding a polypeptide of 1 58 aa with a molecular weight of 1 7.6 kD.Sequence analysis showed that such kind of SHSPs was conserved in different plants and belonged to the cytosolic class I (CI ).The transcriptional level of OsSHSP1 7.6 was significantly up-regulated by heat shock with qRT-PCR.The protein appeared to be accumulated up to higher level under the condition of heat shock in western blotting assays.Taken together, these findings consistently indicated that OsSHPS1 7.6 could be involved into the response to heat shock,which could contribute to understanding the function of OsSHSP1 7.6.关键词
小热休克蛋白/热激处理/表达模式Key words
small heat shock protein (SHSP)/heat shock/expression pattern分类
生物科学引用本文复制引用
项聪英,蔡年俊,李静,羊健,陈剑平,张恒木..一个水稻小热休克蛋白基因的克隆和鉴定[J].中国水稻科学,2016,30(6):587-592,6.基金项目
浙江省自然科学基金资助项目(LQ14-C140003) （LQ14-C140003）
