中国水稻科学2016，Vol.30Issue(6)：603-610,8.DOI:10.16819/j.1001-7216.2016.6033
水稻淡绿叶突变体 HM133的遗传分析与基因定位
Genetic Analysis and Gene Mapping of a Pale Green Leaf Mutant HM133 in Rice
摘要
Abstract
The pale green leaf mutant HM133 was identified from an EMS-induced IR64 mutant bank.The contents of photosynthetic pigments including chlorophyll and carotenoid of HM133 were reduced significantly at 6 weeks and 1 5 weeks after sowing when compared with IR64.The net photosynthetic rate of HM133 was considerably lower than that of the wild-type IR64 at heading stage while the stomatal conductance was apparently increased.The agronomic traits including plant height,number of filled grain per panicle and seed-setting rate decreased significantly in the mutant compared with the wild-type.In addition,the mutant exhibited a less number of grana,irregular arrangement of thylakoid layer in the chloroplast at the tillering stage.Genetic and mapping analysis showed that the pale green phenotype was controlled by a single recessive gene located in the long arm of chromosome 3 between SSR markers RM143 and RM3684.The interval contains an ORF OsChlD encoding magnesium-chelatase D subunit.Sequence analysis revealed that the mutant allele carried a nucleotide substitution from G to A in the tenth exon of OsChlD , which led to the substitution of glutamic acid for arginine acid.Therefore,it is deduced that OsChlD is the candidate gene controlling the pale green leaf phenotype of HM133 .关键词
水稻/淡绿叶/基因定位/镁螯合酶/光合作用Key words
Oryza sativa/pale green leaf/gene mapping/magnesium-chelatase/photosynthesis分类
生物科学引用本文复制引用
施勇烽,贺彦,郭丹,吕向光,黄奇娜,吴建利..水稻淡绿叶突变体 HM133的遗传分析与基因定位[J].中国水稻科学,2016,30(6):603-610,8.基金项目
国家863计划资助项目(2014AA10A603) （2014AA10A603）
浙江省超级稻研究重点实验室项目(2013E10021) （2013E10021）
