中国水稻科学2016，Vol.30Issue(6)：653-660,8.DOI:10.16819/j.1001-7216.2016.6032
褐飞虱 NlTgo 基因的克隆及功能研究
Molecular Cloning and Functional Analysis of N lTgo in the Rice Brown Planthopper,Nilaparvata lugens (Hemiptera:Delphacidae)
摘要
Abstract
Transcriptional regulator Tango (Tgo)plays crucial roles in insect development,including neurogenesis, hematopoiesis,sex determination and gut development.In this study,a full-length cDNA of Tango was cloned in Nilaparvata lugens .qRT-PCR and RNA interference (RNAi)were further used to analyze the expression pattern and function role,respectively.Our results showed that NlTgo contained a 2007-bp open reading frame (ORF),encoding 669 amino acid residues.Sequence alignment showed that NlTgo shared an identity of 68% with Pediculus humanus . Phylogenetic analysis suggested that NlTgo was closely related to the Tango proteins from Acyrthosiphon pisum , P yrrhocoris apterus and Cimex lectularius .Expression profile revealed that NlTgo expression was higher in the first-and second-instar larvae than that in eggs.Furthermore,the expression level of NlTgo was higher in ovary than that in integument.Knocking down of NlTgo ,in the 4th-instar nymph,was performed by double-stranded RNA (dsRNA) targeting NlTgo .It was found that the expression level of NlTgo ,4 days after injection,was significantly decreased by 77%,compared with control.Furthermore,nymphs died due to the abnormal molting,and the survival rate was only 23%,significantly lower than control group (98%).The results suggest that NlTgo is involved in the development of N .lugens and can serve as a potential target for controlling the brown planthopper.关键词
褐飞虱/Tango/RNA 干涉/蜕皮/害虫防治Key words
Nilaparvata lugens/Tango/RNAi/molting/pest control分类
生物科学引用本文复制引用
陈龙飞,万品俊,王渭霞,傅强,朱廷恒..褐飞虱 NlTgo 基因的克隆及功能研究[J].中国水稻科学,2016,30(6):653-660,8.基金项目
现代农业产业技术体系专项(CARS-1-18) （CARS-1-18）
国家自然科学基金资助项目(31201512) （31201512）
