中国动物传染病学报Issue(5)：57-62,6.
夹心ELISA检测牛γ-干扰素方法的建立及应用
DEVELOPMENT AND APPLICATION OF SANDWICH ELISA FOR DETECTING BOVINE INTERFERON GAMMA
摘要
Abstract
To develop a sandwich ELISA for detection of bovine interferon γ (BoIFN-γ), 3 monoclonal antibodies against BoIFN-γ were tested as capture antibodies. All of 3 monoclonal antibodies recognized BoIFN-γ. The sandwich ELISA method was optimized and monoclonal antibody 3E1 was determined as the capture antibody. Total 66 serum samples from different regions were tested using the sandwich ELISA developed here and a commercial ELISA kit. The agreement between these two methods was 95.45%, suggesting the promising possibility of the sandwich ELISA for effective detection of BoIFN-γ in bovine tuberculosis disease.关键词
牛γ-干扰素/双夹心ELISA/应用Key words
Bovine interferon γ/sandwich ELISA/application分类
农业科技引用本文复制引用
钱琨,赵巍,黄大卢,邵红霞,秦爱建..夹心ELISA检测牛γ-干扰素方法的建立及应用[J].中国动物传染病学报,2016,(5):57-62,6.基金项目
江苏省科技支撑计划（BE2013391）；江苏省优势学科项目 （）
