中国动物传染病学报Issue(5)：69-73,5.
2-型猪链球菌保护性抗原ESA的细胞定位及B细胞表位预测
PREDICTION OF CELLULAR LOCALIZATION AND B CELL EPITOPES OF PROTECTIVE ANTIGEN ESA OFSTREPTOCOCCUS SUIS SEROTYPE 2
摘要
Abstract
The cellular location and B cell epitopes of the protective antigen ESA (epidermal surface antigen, ESA) ofStreptococcus suis serotype 2 (S. suis 2) were predicted for the development of a multi-epitope vaccine using bioinformatics. Putative amino sequence of ESA was analyzed using bioinformatics software containing signal peptide and transmembrane region prediction. The secondary structural fl exible regions of ESA were analyzed by using the Garnier-Robson’s method, Chou-Fasman’s method and Karplus-Schulz’s method based on its putative amino sequence. The hydrophilicity plot, surface probability plot and antigenicity index were obtained by using the methods of Kyte-Doolittle, Emini and Jameson-Wolf, respectively. Combined with these results, ESA was predicted as a transmembrane protein and amino acids 30~35, 59~64, 178~185, 245~253 at the N-terminus might represent possible B cell epitopes for ESA. In conclusion, the cellular localization and B cell epitopes of ESA were predicted based on multiple parameters and softwares, which would benefi t design and future research of a multi-epitope vaccine.关键词
2-型猪链球菌/ESA/细胞定位/B细胞表位Key words
Streptococcus suis serotype 2/epidermal surface antigen/localization/B cell epitope分类
农业科技引用本文复制引用
刘丽娜,钟乃凤,王赟,张洁,周静..2-型猪链球菌保护性抗原ESA的细胞定位及B细胞表位预测[J].中国动物传染病学报,2016,(5):69-73,5.基金项目
贵州省科技合作计划 （）
