安徽农业科学2016，Vol.44Issue(31)：1-2,17,3.
里氏木霉纤维素酶水解稻草的研究
Enzymatic Hydrolysis of Rice Straw by Trichoderma reesei Cellulase
摘要
Abstract
Objective] To investigate the effects of carbon and nitrogen sources on cellulase production by Trichoderma reesei and the condi-tions for enzymatic hydrolysis of rice straw.[Method] The fermentation conditions of T.er esei were studied by adding different carbon sources and yeast powder of different concentration.Enzymatic hydrolysis of rice straw was carried out using different amounts of cellulase, and the en-zymatic hydrolysis reaction of rice straw was carried out by cellulase and mixed enzyme of cellulase and xylanase .[ Result] Cellulase activity was high when the compound carbon source of lactose and rice straw and yeast powder concentration of 12 g/L were used.The suitable amount of enzyme was 200 U (FPA ) per gram of rice straw for enzymatic hydrolysis.After 96 h enzymatic hydrolysis of rice straw, the yield of 65.4%was achieved by combination of cellulase and xylanase.[Conclusion] The study provided a certain basis of application of T.reesei cellulase production and enzymatic hydrolysis of rice straw.关键词
纤维素酶/里氏木霉/稻草酶解Key words
Cellulase/Trichoderma reesei/Enzymatic hydrolysis of rice straw分类
生物科学引用本文复制引用
顾斌涛,黄朝,黄国昌,邱小忠,吕珺,张婷..里氏木霉纤维素酶水解稻草的研究[J].安徽农业科学,2016,44(31):1-2,17,3.基金项目
江西省科研院所基础设施配套项目（20142BBA13030）；江西省科学院科研开发专项（2014－YYB －01）；江西省科学院协同创新专项资金普惠一类项目（2013－XTPH－29）。 （）
