安徽农业科学2016，Vol.44Issue(31)：5-7,3.
不同培养基和储存条件对家蝇幼虫分泌物中溶菌酶含量的影响
Effects of Different Culture Media and Storage Conditions on the Lysozyme Content in the Secretions of Housefly Larvae
摘要
Abstract
Objective] To study the effects of different culture media and storage conditions on lysozyme content in the secretions of housefly larvae.[ Method] Swine manure and chicken manure were used as the culture media to feed housefly larvae .The housefly larvae in control group were fed with wheat bran and that in induced group were fed with wheat bran with adding Escherichia coli.The secretions of 2-instar and 3-instar housefly larvae in each group were extracted to determine lysozyme content in the secretions by using reagent kits .The secretions in 3-instar larvae in control group and induced group were extracted to analyze the changes of lysozyme content at room temperature , 4 ℃ and-20 ℃ respectively.[ Result] The average lysozyme content in the secretions of 3-instar larvae in swine manure group, chicken manure group,control group and induced group were 2.462,2.782,1.019 and 2.117 μg/mL respectively, significantly higher than that in 2-instar lar-vae(P<0.05).The lysozyme content in the secretions of 2-instar larvae and 3-instar larvae in swine manure group,chicken manure group and induced group were higher than that in control group(P<0.05).At room temperature, lysozyme activity in control group and induced group could be maintained for about 14 days.At -20 ℃, lysozyme activity could be maintained for about 70 days.At 4 ℃,the maintenance time of lysozyme activity in control group and induced group were in between room temperature and -20 ℃, and lysozyme content in these two groups had some differences at different time .[ Conclusion] The lysozyme content in the in the secretions of 3-instar larvae is higher than that in 2-instar larvae.Swine manure culture medium and chicken manure culture medium can significantly increase the lysozyme content in the se-cretions of 2-instar larvae and 3-instar larvae.And higher lysozyme activity in housefly larvae can be maintained at -20 ℃.关键词
家蝇/溶菌酶/猪粪/鸡粪Key words
Housefly/Lysozyme/Swine manure/Chicken manure分类
农业科技引用本文复制引用
王建宇,国果,杨蔚锦,陶如玉,陈亚..不同培养基和储存条件对家蝇幼虫分泌物中溶菌酶含量的影响[J].安徽农业科学,2016,44(31):5-7,3.基金项目
国家自然科学基金项目（81160204）；高校博士点学科专项科研基金项目（20105215120001）；国家级大学生创新训练项目（201310660016）。 （）
