安徽农业科学2016，Vol.44Issue(31)：34-36,39,4.
蜂蜜中磺胺类和氯霉素类同时测定的前处理方法研究
Study on Pretreatment Method for Simultaneous Determination of Chloramphenicol and Sulfanilamide Grous in Honey
仲光凤 1刘晓玲2
作者信息
- 1. 山东中质华检测试验检验有限公司，山东济宁272000
- 2. 济宁市食品药品检验检测中心，山东济宁272007
- 折叠
摘要
Abstract
Objective] To establish a new method UPLC-MS for the determination of sulfanilamide grous and chloramphenicols in honey . [Method] With 0.2% formic acid acetonitrile as the extraction agent, through purification treatment by a new column ( Oasis® PRiME HLB), sulfanilamide grous and chloramphenicols can be extracted simultaneously.[Result] Chloramphenicol had a good linear relationship in the range of 0.1-10.0 ng/mL, and the correlation coefficient was greater than 0.991; sulfanilamide grous had a good linear relationship in the range of 0.5 -50.0 ng/mL.The lowest detection limit of chloramphenicol was 0.1 g/kg, detection limit of sulfanilamide grous was 0.5 μg/kg, recovery was 82.5%-101.2%, relative standard deviation was 3.2% -4.0%.[Conclusion] The method is rapid, accurate and sensitive, and it is suitable for the detection of chloramphenicol and sulfanilamide grous.关键词
超高效液相色谱串联质谱/磺胺类/氯霉素类/蜂蜜Key words
UHPLC-MS/Sulfanilamide grous/Chloramphenicols/Honey分类
农业科技引用本文复制引用
仲光凤,刘晓玲..蜂蜜中磺胺类和氯霉素类同时测定的前处理方法研究[J].安徽农业科学,2016,44(31):34-36,39,4.
