安徽农业科学2016，Vol.44Issue(31)：153-155,3.
小叶朴组织培养无菌体系的建立
The Establishment of Tissue Culture Sterile System of Celtis bungenana
王斯彤1
作者信息
- 1. 辽宁省固沙造林研究所，辽宁阜新123000
- 折叠
摘要
Abstract
Objective] The aim was to establish tissue culture sterile system of Celtis bungenana.[Method]Through the comparison of differ-ent disinfectants and disinfection time, tissue culture sterile system of Celtis bungenana with lowerpollution rate and higher germination rate were screened.[ Result] By comparison with 70% alcohol disinfection 30 s 2 times, with mercuric chloride for 6 minutes from the stem seg-ments were of low pollution, high germination rate.Late for the fungus pollution using carbendazim as disinfectant and additives, soaking with 200 mg/L carbendazim explant 60 min, can effectively control the pollution, and can have better germination rate.200 mg/L is added in the culture medium carbendazim can restrain fungus contamination, and better than carbendazol soaking process.[ Conclusion] The study stabli-hed tissue culture sterile system of Celtis bungenana,and provide reference for proliferation and rooting of Cetlis bungenana.关键词
小叶朴/消毒处理/组织培养Key words
Celtis bungen ana/Disinfection treatment/Tissue culture分类
农业科技引用本文复制引用
王斯彤..小叶朴组织培养无菌体系的建立[J].安徽农业科学,2016,44(31):153-155,3.
