北京中医药大学学报2016，Vol.39Issue(11)：902-908,7.DOI:10.3969/j.issn.1006-2157.2016.11.004
健脾化湿通络方对佐剂关节炎大鼠滑膜血管新生的影响
Effect of Jianpi Huashi Tongluo Formula on synovial angiogenesis in rats with adjuvant arthritis
摘要
Abstract
Objective To observe the mechanism of Jianpi (invigorating the spleen)Huashi (resovling dampness)Tongluo (removing obstruction in collaterals)Formula (JHTF)on PI3K/AKT/mTOR signa-ling pathway,hypoxia inducible factor (HIF-1α),vascular endothelial growth factor (VEGF-A),mi-crovessel density (MVD)and endostatin (ES)of synovial tissue in rats with adjuvant arthritis (AA). Methods Fifty rats were randomly divided into five groups — normal control group,model group, methotrexate group,tripterygium glycosides tablet group and JHTF group.Rat AA model was induced by using Freund's complete adjuvant.After modeling,each treatment group were i.g.corresponding medica-tion for consecutive 30 days,and then the expression of microvascular density (MVD)was detected using immunohistochemical,interleukin (IL)-6,IL-10,HIF-1αand VEGF were measured by enzyme-linked immunosorbent assay,and PI3K,AKT1,p-AKT1,mTOR and ES proteins were detected by Western blotting.Results Compared with normal control group,footpad swelling,arthritic index increased,ser-um IL-6,VEGF and HIF-1αand MVD,PI3K,AKT1,p-AKT1,mTOR,ES in synovial vascular signifi-cantly increased in the model group(P <0.05 or P <0.01).Compared with the model group,the MVD count,serum VEGF-A,HIF-1αand IL-6 decreased in JHTF group.The expressions of PI3K,p-AKT1, mTOR,ES in synovial vascular in JHTF group were lower ,and serum IL-10 was higher than those of the model control group.Conclusion Jianpi Huashi Tongluo Formula can improve angiogenesis by regula-ting PI3K/AKT/mTOR pathway,expression of HIF-1αand ES in rat AA model.关键词
健脾化湿通络方/佐剂关节炎/血管新生/PI3K/AKT/mTOR 通路/大鼠Key words
Jianpi Huashi Tongluo Formula/adjuvant arthritis/angiogenesis/PI3K/AKT/mTOR path-way/rats分类
医药卫生引用本文复制引用
张晓军,刘健,万磊,黄传兵..健脾化湿通络方对佐剂关节炎大鼠滑膜血管新生的影响[J].北京中医药大学学报,2016,39(11):902-908,7.基金项目
国家自然科学基金青年项目（No．81403388），安徽省科技厅项目（No．604 f0804030），安徽省自然科学基金项目（No．1508085QH159），安徽省重点实验室项目（No．1606c08238），安徽省公益性技术应用研究联动计划项目（No．1604 f0804030），安徽中医药大学校级基金项目（No．2014qn025）。 （）
