北京中医药大学学报2016，Vol.39Issue(11)：909-914,6.DOI:10.3969/j.issn.1006-2157.2016.11.005
健脾益气化瘀解毒方含药血清对结肠癌 HCT116细胞增殖、周期、凋亡的影响
Effect of Jianpi Yiqi Huayu Jiedu Formula on proliferation,cycle and ap-optosis of HCT116 colorectal cancer cells
摘要
Abstract
Objective To study the effect of medicated serum of Jianpi Yiqi Huayu Jiedu Formula (JY-HJF),a TCMcompound formulated according to TCMprinciple of tonifying spleen,replenishing qi,re-moving blood stasis and detoxification,on proliferation,cycles,apoptosis of HCT116 colorectal cancer cells and on the protein expression of Caspase-3,Bcl-xL and Bax of HCT116 cells.Methods After drug-contained serum (10%,15%,20%)treated HCT116 cells for 12,24,48 and 72 h,the prolifera-tion was detected by MTT method;after treated for 24 or 48 h,the cell cycle and apoptosis rate were de-tected by propidium iodide (PI)staining.The protein expression level of Caspase 3,Bcl-xL and Bax in HCT116 cells were detected by Western blot.The control group was treated by blank serum and 10% fe-tal bovine serum (FBS).Results Compared with the same concentration of blank serum group,the cell proliferation was inhibited significantly in 15% and 20% medicated serum group after 12 h (P <0.05), and the inhibitory effect showed time-and concentration-dependence with the highest at 72 h (P <0. 05).In 10% and 15% medicated serum group,cells in G2 phase decreased,but the G1-phase cells and S-phase cells increased;cells in G1 phase and S phase increased and those in G2 phase decreased in 10% medicated serum group with the remarkable results at 48 h.It was suggested that the medicated ser-um could blocked cell cycles,with the positive correlation with time.Compared with the blank serum groups at the same concentration,after treated 24 and 48 h,apoptosis rate of HCT116 cells increased, and had the positive correlation with time and concentration.Compared with the blank serum group,the expression of Bax protein was upregulated and that of Caspase-3 and Bcl-xL were downregulated in 15%medicated serum group (P <0.05).Conclusion The effect of Jianpi Yiqi Huayu Jiedu Formula on in-hibiting proliferation and inducing apoptosis may be via the way of blocking HCT116 cells cycles and of upregulating Bax protein expression with downregulating Caspase-3 protein and Bcl-xL protein expression.关键词
健脾益气化瘀解毒/HCT116 细胞/增殖/细胞周期/凋亡Key words
Jianpi Yiqi Huayu Jiedu Formula/HCT116 colorectal cancer cells/proliferation/cell cy-cle/apoptosis分类
医药卫生引用本文复制引用
简小兰,杨晓,罗吉,李勇敏,吕元,谭小宁,蒋益兰..健脾益气化瘀解毒方含药血清对结肠癌 HCT116细胞增殖、周期、凋亡的影响[J].北京中医药大学学报,2016,39(11):909-914,6.基金项目
国家自然科学基金资助项目（No．81373602），湖南省研究生科研创新项目（No．CX2015B319），湖南省中医药研究院项目 （）
