北京中医药大学学报2016，Vol.39Issue(11)：915-919,5.DOI:10.3969/j.issn.1006-2157.2016.11.006
七龙天对低氧性肺动脉高压大鼠 IL-1β、TNF-α的影响
Effect of Qilongtian on IL-βand TNF-αin hypoxic pulmonary hyperten-sion rats
摘要
Abstract
Objective To study the effects of Qilongtian on interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)in hypoxic pulmonary hypertension (HPH)rats.Methods All male rats were ran-domly divided into six groups:normal group,model group,bosentan group,Qilongtian high-,mid-and low-group.Except the rats in normal group,those in other five groups were under atmospheric hypoxia to establish HPH model.From the first day of modeling,all medication groups were i.g corresponding drugs for consecutive three weeks.Pulmonary hemodynamic parameters were detected by right heart catheteriza-tion under direct vision,mRNA expression of IL-1βand TNF-αin lung tissue were measured using ELISA,protein expression of IL-1βand TNFαin lung tissue were measured using immunohistochemistri-cal methods.Results Qilongtian and bosentan significantly reduced the level of mean pulmonary arterial pressure (MPAP),IL-1βand TNF-αexpression in HPH rats,and the effect of Qilongtian high dosage was stronger with the dose-dependence.Compared with normal group,expression of IL-1βand TNF-αmRNA and protein increased,and those in model group and bosentan group did significantly(P <0.01). Compared with the model group,there was no significant difference in bosentan group (P >0.05),the expression level of IL-1βand TNF-αwere significantly downregulated in all Qilongtian groups (P <0.01 or P <0.05).Compared with the bosentan group and all the Qilongtian groups,there were significant differences,and the Qilongtian high-,mid-dose group had more impact (P <0.01 or P <0.05).Con-clusion High,middle and low dosage of Qilongtian reduced pulmonary hypertension,with high dosage prior to bosentan.The mechanism of lower blood pressure may be via downregulating the expression of IL-1βand TNF-α,but it would be influenced by other vascular tension factors and angiogenic factors.关键词
七龙天/肺动脉高压/白细胞介素-1β/肿瘤坏死因子-α/大鼠Key words
Qilongtian/hypoxic pulmonary hypertension/interleukin-1β/tumor necrosis factor-α/rats分类
医药卫生引用本文复制引用
冷萍,余晓玲,魏丹霞,苏婷,付义..七龙天对低氧性肺动脉高压大鼠 IL-1β、TNF-α的影响[J].北京中医药大学学报,2016,39(11):915-919,5.基金项目
国家自然科学基金资助项目 （）
