吉林大学学报（医学版）2016，Vol.42Issue(6)：1045-1048,4.DOI:10.13481/j.1671-587x.20160601
钙离子在顺铂诱导宫颈癌HeLa细胞自噬反应中的作用
Role of Ca2+ in cisplatin-induced autophagy of cervical cancer HeLa cells
摘要
Abstract
Objective:To treat the cervical cancer HeLa cells by using calcium inhibitors combined with chemotherapy drugs,and to explore the effect of Ca2+ on autophagy in cancer cells,and to provide basis for tumor treatment and drug research.Methods:The human cervical cancer HeLa cells were at logrithmic growth phase randomly divided into control group, cisplatin group, 2-APB group, and cisplatin + 2-APB group (combination group).MTT assay was used to measure the survival rates of HeLa cells;Ca2+-sensitive fluorescent dye was used to detect the levels of Ca2+ in cytoplasm and mitochondrial;the colocalization of LC3 and P62 were examined under confocal microscope.Results:The MTT assay results showed that compared with control group,the survival rates of HeLa cells in cisplatin and combination groups were signifcantly decreased (P<0.05).Compared with cisplatin group,the survival rate of HeLa cells in combination group was significantly increased (P<0.05).The Ca2+-sensitive fluorescent dye detection results showed that compared with cisplatin group,the mitochondrial free Ca2+level and the fluorescence intensity of colocalization of LC3 and P62 protein in combination group were significantly decreased.Conclusion:Chemotherapy drugs can induce the autophagy of tumor cells, and calcium inhibitors can increase the sensitity of tumor cells to chemotherapy drugs by reducing the autophagy levels.关键词
顺铂/钙离子/自噬/宫颈肿瘤Key words
cisplatin/calcium ion/autophagy/cervical neoplasms分类
医药卫生引用本文复制引用
徐路,曾林川,于洋,窦茗瀚,刘师兵,李松岩,徐冶..钙离子在顺铂诱导宫颈癌HeLa细胞自噬反应中的作用[J].吉林大学学报（医学版）,2016,42(6):1045-1048,4.基金项目
国家自然科学基金面上项目资助课题（81372793）；吉林省教育厅“十三五”科技项目资助课题（2016237）；吉林省大学生创新创业项目资助课题 （）
