吉林大学学报（医学版）2016，Vol.42Issue(6)：1054-1058,5.DOI:10.13481/j.1671-587x.20160603
Mu2蛋白亚细胞结构定位及其相互作用蛋白的筛选
Subcellular localization of Mu2 protein and screening of its interacting proteins
摘要
Abstract
Objective:To express the FLAG-Mu2 protein in drosophila S2 cells,and to investigate its subcellular localization and to purify and identify the FLAG-Mu2 protein complex, and to provide basis for the study of Mu2 protein. Methods:The vector was transfected into drosophila S2 cells. The subcellular localization of FLAG-Mu2 protein was tested by confocal laser scanning microscope. The expression levels of FLAG-Mu2 protein in nuclear extractions were examined using nuclear and cytoplasmic protein extraction method. The FLAG-Mu2 complex was preciptated by precipitation technology and purified by chromatographic steps and the components were analyzed by mass spectrometry. Results:The confocal laser scanning microscope results showed that the FLAG-Mu2 protein mainly expressed in nucleus.The nuclear and cytoplasmic protein extraction results showed that the fusion protein, with a relative molecular weight of 140 000,mainly existed in nuclear extraction. The FLAG-Mu2 complex was purified and analyzed by mass spectrometry,and its results showed that there were 5 components,including pif1A, CG31782,mip120,CG31690,and G protein-sa60A,may be included in FLAG-Mu2 complex. Conclusion:The FLAG-Mu2 protein mainly localizes in the nucleus of drosophila S2 cells;a total of 5 kinds of proteins are found to be potential components of FLAG-Mu2 protein complex.关键词
Mu2 蛋白/融合蛋白/亚细胞结构定位/相互作用蛋白筛选Key words
Mu2 protein/fusion protein/subcellular localization/screening of interacting proteins分类
生物科学引用本文复制引用
霍云龙,王春玉,赵越..Mu2蛋白亚细胞结构定位及其相互作用蛋白的筛选[J].吉林大学学报（医学版）,2016,42(6):1054-1058,5.基金项目
国家自然科学基金资助课题（31271364，31401115）；国家重点基础研究发展计划（973计划）项目资助课题（2013CB945200）；教育部科学技术研究项目资助课题 （）
