吉林大学学报（医学版）2016，Vol.42Issue(6)：1243-1248,6.DOI:10.13481/j.1671-587x.20160638
四氯化碳诱导的肝硬化小鼠肝部分切除后肝再生模型的建立及评价
Establishment of liver regeneration model of cirrhosis mice induced by CCl4 after partial hepatectomy and its evaluation
摘要
Abstract
Objective:To establish and evaluate the model of liver regeneration of the cirrhosis mice after partial hepatectomy.Methods:Fifty mice were randomly divided into experiment group (n=25)and control group (n=25).The mice in experiment group were injected with CCl4 intraperitoneally twice per week for 8 weeks and the mice in control group were inj ected with soya oil. Partial hepatectomy of 3 7% liver was performed on all mice in two groups.The successful rate of operation,survival rate after operation,survival statue after operation and postoperative liver regeneration rate of the mice in two groups were observed;the pathologic characteristics of liver tissue of the mice in two groups were observed by HE staining and Masson staining;the levels of serum hepatocyte growth factor (HCG)and alanine aminotransferase (ALT)of the mice in two groups were detected by ELASA methods.Results:The successful rates of operation of the mice in two groups were both 100%,and the survival rates of the mice at 6 weeks after operation were 100%.During 3 weeks after operation,the regeneration rates of liver tissue of the mice in experiment group were lower than those in control group;the regeneration rate of liver tissue of the mice in experiment group was equal to that in control group at 3 weeks after operation;the regeneration rates of liver tissue of the mice in experiment group were higher than those in control group from 3 weeks after operation.The HE staining and Masson staining results showed that the liver tissue presented the pathological changes of cirrhosis 8 weeks after inj ection with CCl4;after partial hepatectomy,the structures of liver tissue were gradually improved.The levels of serum HGF of the mice in experiment group were significantly higher than those in control group;the levels of serum HGF of the mice in two groups were increased compared with the preoperative levels,and then recovered to the preoperative level.Compared with control group,the levels of serum ALT of the mice in experiment group after hepatectomy were increased significantly,and the duration was longer and the time to reach the peak was delayed.Conclusion:It is extremely easy to establish a stable and reliable liver regeneration model of liver cirrhosis mice after partial hepatectomy by the resection of left lateral lobe of the cirrhosis mice induced by CCl4 with silk ligation.关键词
肝硬化/小鼠, ICR/肝切除/四氯化碳/疾病模型,动物Key words
cirrhotic liver/mice,ICR/hepatectomy/carbon tetrachloride/disease model,animal分类
医药卫生引用本文复制引用
陈阳,鄂长勇,关连越,刘文韬,王丽娜,张学文..四氯化碳诱导的肝硬化小鼠肝部分切除后肝再生模型的建立及评价[J].吉林大学学报（医学版）,2016,42(6):1243-1248,6.基金项目
吉林省科技厅自然科学基金资助课题（201215079）；吉林大学白求恩基金资助课题 （）
