发光学报2016，Vol.37Issue(12)：1560-1565,6.DOI:10.3788/fgxb20163712.1560
ITC研究达卡巴嗪与DNA反应动力学
Interaction Dynamics Between Dacarbazine and DNA
摘要
Abstract
The interaction between calf thymus DNA and anti-cancer drug dacarbazine was investiga-ted using isothermal titration calorimetry( ITC) , spectroscopy, viscosity and other methods. The hy-perchromic effect and blue shift in the absorption spectra as well as viscosity decline are observed clearly after the interaction between dacarbazine and DNA. Furthermore, the binding constant and number of binding sites are also recorded by ITC, presenting that the interactions of the dacarbazine with DNA can be categorized for two modes: a non-classical intercalation type and surface binding effect. In former,△H1 <0, △S1 >0, K1 =5. 63 × 104 , the binding site is 0. 10. In latter, only surficial interaction during the combination of the drug molecule with DNA occurs, not embedding into the hydrophobic part of the DNA molecule, due to△H2 > 0 ,△S2 > 0, K2 =1. 00 × 103 , and the binding site of 9. 99. Meanwhile, UV method is also employed to find the binding constant of Ka=6. 70 × 104 , which is consistence with ITC observation.关键词
DNA/达卡巴嗪/光谱法/作用机理/等温滴定量热Key words
DNA/dacarbazine/spectroscopy/interaction mechanism/isothermal titration calorimetry分类
化学化工引用本文复制引用
王欢,王姣,李宗孝,赵微微,蒲小华,程花蕾..ITC研究达卡巴嗪与DNA反应动力学[J].发光学报,2016,37(12):1560-1565,6.基金项目
国家自然科学青年科学基金(21501007)；陕西省自然科学基金(2012Jm2019)；陕西省教育厅项目(14JK1045)资助 （21501007）
