广西植物2016，Vol.36Issue(11)：1282-1287,6.DOI:10.11931/guihaia.gxzw201604020
温度对马尾松组培单芽不定根发生的影响
Effects of temperature on adventitious root formation of tissue-cultured shoots in Pinus massoniana
摘要
Abstract
Pinus massoniana is one of the most important tree species for afforestation in South China, and it is of great economic value and ecological benefit. P. massoniana is famous for the wide application of its timber and resin, being of very important high-value tree species. Breeding programs for P. massoniana were initiated in the 1980s in China. To capture the best genetic stock from the breeding program, an efficient system for rapid clonal propagation is of considera-ble value. Therefore, the application of biotechnology and especially tissue culture provides an important tool to propa-gate the selected genotypes. The regeneration of plants under aseptic and controlled environmental conditions is referred to as micropropagation because very small pieces of plant tissue or organs are used as starting vegetative tissue. Previousstudies revealed that P. massoniana was hard to root by tissue culture, and culture temperature was correlated to the sta-bility of in vitro rooting in P. massoniana. In the illumination incubator with strictly controlled conditions of light and temperature, variations of rooting ability index, including rooting percentage, rooting time, number of roots and trans-plantation survival percent, and rooting anatomic structure from in vitro single buds were investigated using subcultured shoots of Pinus massoniana clone under rooting treatments of different temperatures. SPSS 19.0 statistic software was used to analyze those data. In vitro rooting performance was good and the highest transplantation survival percentage was 98.1% for shoots of P. massoniana cultured at 25℃. Rooting time was prolonged, number of roots was decreased and rooting percent was low under the lower temperature treatment, while rhizome callus was serious and transplantation sur-vival percent remarkably was reduced under the higher temperature treatment. For P. massoniana, vascular cambium cells were differentiated into adventitious root in terms of root anatomical structure. Compared with the normal tempera-ture treatment at 25℃, cell division was passive, induced adventitious root primordial at 20 ℃ treatment, however, cells were active, the color of stained cells from pith ray and vascular tissue was deepened and those cells were arranged densely, resulting in induced adventitious root primordial was separated into being zonal or schistose at 30 ℃treatment. This study revealed the effects of temperature on in vitro rooting ability, and provides the supports for the in-dustrial production of tissue cultured plantlets in P. massoniana.关键词
组培快繁/生根率/生根解剖/不定根诱导Key words
rapid propagation by tissue culture/rooting percentage/rooting anatomy/induction of adventitious root分类
农业科技引用本文复制引用
姚瑞玲,王胤..温度对马尾松组培单芽不定根发生的影响[J].广西植物,2016,36(11):1282-1287,6.基金项目
广西优良用材林资源培育重点实验室自主研究课题(13A-01-01)；广西科学研究与技术开发计划项目(桂科合14125008-2-17，桂科攻1598006-5-7)；广西林业科技重点项目(桂林科研[2015]7号)；国家自然科学基金(31360178)[Supported by the Independent Program from the Key La-boratory of Gaungxi Fine Timber Forest Resources Cultivation (13A-01-01)；the Program of Science Research and Technology Development from the Depart-ment of Science and Technology of Guangxi (14125008-2-17，1598006-5-7)；the Key Program of Guangxi Forestry Bureau ([2015]7)；the National Natural Science Foundation of China (31360178)]。 （13A-01-01）
