广西植物2016，Vol.36Issue(11)：1295-1302,8.DOI:10.11931/guihaia.gxzw201508018
外源激素对马尾松花芽分化及内源物质的影响
Effects of exogenous hormones on flower bud differentiation and endogenous substances of Pinus massoniana
摘要
Abstract
In order to investigate the relationship between the formation of Pinus massoniana cones plant hormone levels, This paper carried out a series of hormone treatments by means of injecting IAA, IBA, GA3, BAP, etc. to the 11 years old P. massoniana in Duyun P. massoniana Clonal Seed Orchard, and then the changes of soluble protein and the con-tent of soluble sugar were measured under different concentrations of different hormones using Coomassie Brilliant Blue G-250 staining and anthrone method respectively. And the bloom of the test branches was investigated in the second year. The experimental results were as follows:Firstly, in August to November, the BAP with 500 mg·L-1 was condu-cive to the formation of male and female cones of P. massoniana. Secondly, the GA3 with 100 mg·L-1 was conducive to the formation of female cones of P. massoniana. Thirdly, both GA3 with 250 mg·L-1 and GA3 with 500 mg·L-1 were conducive to the formation of male cones of P. massoniana. Fourthly, the IAA with 250 mg·L-1 was conducive to in-crease the number of male and female cone with the same branch. Finally, treating the masson by injecting BAP, IAA and GA3 with 500 mg·L-1 respectively in October and November could have a significant impact on the changes of pro-tein content in needles of P. massoniana. Finally, In October, using the concentration of BAP 100 mg·L-1 of the bran-ches to process their soluble sugar content and soluble protein content could reach a significant level. Respectively in Au-gust and October, with a concentration of 100 mg·L-1 IBA and concentration of IBA 250 mg·L-1 processed soluble pro-tein content and its control was in a very significant difference, In November, using the concentration of 100 mg·L-1 GA3 processed soluble protein content and its control was in a very significant difference. While in November using the concentration of IBA 500 mg·L-1 , soluble protein content and the control difference were significant.关键词
马尾松/外源激素/雌雄球花分化/内源物质Key words
Pinus manssoniana/exogenous hormones/male and female cones differentiation/endogenous substances分类
农业科技引用本文复制引用
陈庭巧,赵杨,秦雪,朱亚艳,王秀荣..外源激素对马尾松花芽分化及内源物质的影响[J].广西植物,2016,36(11):1295-1302,8.基金项目
国家自然科学基金(3146020)；贵州省重大科技专项([2012]6011-2)；贵州大学引进人才科研基金[贵大人基合字(2012)031号]；贵州大学研究生创新基金(研农2015001)[Supported by the National Natural Science Foundation of China (3146020)；Major Science and Technology Projects in Guizhou Province ([20126011-2)；Guizhou University Graduate Innovation Fund (2015001)； Introduction of Talent Guizhou University Re-search Foundation (2012-031)]。 （3146020）
