广西植物2016，Vol.36Issue(11)：1396-1402,7.DOI:10.11931/guihaia.gxzw201506007
油樟内生溶磷菌的筛选及其生物学特性
Screening and characterization of phosphate dissolving endophytic bacteria from Cinnamomum longepaniculatum
摘要
Abstract
Screening endophytic strains with phosphate-dissolving and exploring the characterization of growth promo-ting and resistance were conducive to broad the phosphate dissolving microbial resources, to develop microbial fertiliz-ers, to improve soil phosphorus nutritious and to increase agricultural yield. We isolated 50 endophytic bacteria from interior tissues of Cinnamomum longepaniculatum, and 24 phosphate dissolving endophytic bacteria strains were screened using phosphate solubilizing zone ( PKO) inorganic culture medium, subsequently, the capacity of dissolving phosphorus of 24 strains were determined by phosphor-molybdate blue color methods, and the characteristics benefited to plant-microbe interaction-indoleacetic acid ( IAA) , siderophore, 1-aminocyclopropane-1-carboxylic acid deaminase ( ACC) and chitosanase activities were also evaluated. The results showed that 24 strains could release phosphate from tricalcium phosphate with the range of dissolving phosphate from 51.26 μg·mL-1 to 237.0 μg·mL-1, and the maxi-mum phosphate content in the solution was obtained with strain YG60. Among 24 strains, 6 strains ( YG60, YG43, YG36, YG25, YG49, YG44 ) showed the higher phosphate solubilization capacity ( 237. 08, 211. 53, 180. 68, 166.85, 151.28, 150.20 μg·mL-1 respectively) . Furthermore, 24 strains could decrease the pH of the medium with the pH value range from 4.5 to 5.5, and the relationship between pH and phosphorus solubizition was also discussed;however, there were no close relationship between them. The further experimental results showed that most of the 6 strains with higher phosphate dissolving capacity had the ability to produce IAA and siderophores, and had ACC deam-inase and chitosanase activity. Among them, YG43, YG60 and YG25 possessed higher IAA producing capacity (22.55, 18.75 and 16.41 μg·mL-1 respectively); YG43 and YG60 possessed higher siderophores of As/Ar<0.6 (0.459, 0.579 respectively );YG43, YG60 and YG25 had strong ACC deaminase activity (0.194, 0.224, 0.208 U·mg-1),YG43 and YG60 had strong chitosanase activity (2.968 U,2.502 U). In terms of all the properties of dis-solving phosphate, secreting IAA, siderophores, ACC deaminase and chitosanase, strains YG43, YG60 and YG25 isolated from interior tissues of C. longepaniculatum have abundant biological characteristics related to plant growth promotion, stress homeostasis regulation and biocontrol activity. They are possible to be further developed as excellent strains for application.关键词
溶磷内生菌/溶磷能力/IAA/铁载体/ACC脱氨酶/几丁质酶Key words
phosphate-dissolving endophytic bacteria/capacity of dissolving phosphorus/IAA/siderophores/ACC deaminase/chitosanase分类
生物科学引用本文复制引用
冯瑞章,周诰均,魏琴,周万海,范轶玲,秦欢..油樟内生溶磷菌的筛选及其生物学特性[J].广西植物,2016,36(11):1396-1402,7.基金项目
宜宾市科技创新专项(2012ZNY006)；四川省大学生创新创业训练计划项目(20151064b17)；四川省高校科研创新团队项目(14TD0031)；四川省青年科技创新研究团队培育计划项目(2011JTD0035)[Supported by Program for Science and Technology Bureau of Yibin City (2012ZNY006)；Training Programs of Innovation and Entrepreneurship Undergraduates in Sichuan Province (201510641017)； Scientific Research Fund of Sichuan Provincial Education Department (14TD0031)；Sichuan Youth Science and Technology Innovation Teams (2011JTD0035)]。 （2012ZNY006）
