湖南中医药大学学报2016，Vol.36Issue(11)：18-21,4.DOI:10.3969/j.issn.1674-070X.2016.11.005
跌打通痹膏对兔膝骨关节炎关节软骨MMP-13、Ⅱ型胶原mRNA表达的影响
The Effects of Dieda Tongbi Emplastrum on MMP-13, Collagen II mRNA Expression in the Knee Osteoarthritis Model Rabbits
摘要
Abstract
Objective To observe the effects of Dieda Tongbi emplastrum on MMP-13 (matrix metalloproteinase-13), COL-II mRNA expression in the knee osteoarthritis model rabbits. Methods The 65 healthy New Zealand rabbits were randomly divided into normal control group, sham-operation group, modeling group, Dieda Tongbi emplastrum group, Gutong patch group, 13 rabbits in each group. The model group, Dieda Tongbi emplastrum group, Gutong emplastrum group were built by improved Hulth molding method. The normal group and sham-operation group were opened the joint cavities without any treatment. The model group, Dieda Tongbi emplastrumand and Gutong patch group were treated with adhesive tape, Dieda Tongbi emplastrumand and Gutongtie for 4 weeks, respectively. The changes of joint cartilage in all model rabbits were observed, and the expressions of MMP-13 and COL-ⅡmRNA in joint cartilage were detected. Results (1) The histologic articular cartilage score of Dieda Tongbi emplastrum group was lower than model group (P<0.05). (2) MMP-13 mRNA test: the MMP-13 expression in model group was increased than that in normal control group (P<0.01). The MMP-13 mRNA expression of Dieda Tongbi emplastrum group were lower than that in model group (P<0.01). (3) COL-ⅡmRNA test: the expression of COL-Ⅱ in Dieda Tongbi emplastrum group was increased than that in model group (P<0.01). Conclusion Dieda Tongbi emplastrum can protect the articular cartilage in knee osteoarthritis model rabbits and delay the course of knee joint degeneration by reducing COL-Ⅱdegradation and inhibiting the expression of MMP-13.关键词
膝骨性关节炎/跌打通痹膏/MMP-13mRNA/Ⅱ型胶原mRNAKey words
knee osteoarthritis/Dieda Tongbi emplastrum/MMP-13mRNA/CollagenII mRNA分类
医药卫生引用本文复制引用
龚志贤,罗凌威,卢敏,邝高艳,范杰,银丝丝..跌打通痹膏对兔膝骨关节炎关节软骨MMP-13、Ⅱ型胶原mRNA表达的影响[J].湖南中医药大学学报,2016,36(11):18-21,4.基金项目
湖南省教育厅优秀青年科研课题项目(13B085)。 （13B085）
