湖南中医药大学学报2016，Vol.36Issue(11)：22-26,5.DOI:10.3969/j.issn.1674-070X.2016.11.006
苦参碱逆转人结肠癌细胞株（HT-29）奥沙利铂耐药性的作用及机制研究
The Reversal Effects of Matrine on the Multi-drug Resistance of Oxaliplatin in Human Colon HT-29 Cells and its Mechanism Research
摘要
Abstract
Objective To study of matrine on the reversal of multi-drug resistance in HT-29/OXA cells and explore its underlying mechanisms. Methods Oxaliplatin resistant HT-29/OXA cells were established by gradually increasing the concentration of medicines. The reverse efficacy of Matrine on HT-29/OXA cells was determined by CCK-8 assay. The apoptosis and cell cycle were detected by flow cytometry. The expression levels of lung resistance protein (LRP) and P-glycoprotein (P-gp) mRNA and proteins was determined by quantitative real time PCR (qRT-PCR) and Western blot, respectively. Results Matrine can increase the sensibility of HT-29/OXA to oxaliplatin and reverse the part resisitance. Matrine (0.5 μg/mL) and matrine (3.0 μg/mL) alone had no effect on the cell cycle and apoptosis in HT-29/OXA cells. The oxaliplatinin combination with matrine significantly inhibited cell proliferation and induced apoptosis. Oxaliplatin (0.5 μg/mL) and matrine (3.0 μg/mL) alone had no effect on the expression level of LRP and P-gp mRNA and protein in HT-29/OXA cells. The expression of LRP and P-gp mRNA and protein was lower with drug combination. Conclusion Matrine could partially reverse the multi-drug resistance of HT-29/OXA cells to oxaliplatin, which may be related to the lower expression levels of LRP and P-gp.关键词
结肠癌/HT-29/奥沙利铂/多药耐药性/苦参碱Key words
colon cancer/HT-29/oxaliplatin/multi-drug resistance/matrine分类
医药卫生引用本文复制引用
张冬梅,张雅明..苦参碱逆转人结肠癌细胞株（HT-29）奥沙利铂耐药性的作用及机制研究[J].湖南中医药大学学报,2016,36(11):22-26,5.基金项目
上海市进一步加快中医药事业发展三年行动计划资助（2014-2016年，编号ZY3-CCCX-1-1008）。 （）
