吉林大学学报（理学版）2016，Vol.54Issue(6)：1456-1461,6.DOI:10.13413/j.cnki.jdxblxb.2016.06.48
一步法快速分离鉴定肿瘤干细胞的单细胞微流控芯片
Single Cell Microfluidic Chip for Rapid Isolation and Identification of Tumor Stem Cells by One Step
摘要
Abstract
Using multilayer soft lithography technology,poly-dimethylsiloxane (PDMS)as the chip material,we made a novel microfluidic chip (60 mm×40 mm×4 mm)for single cell identification research.We enriched tumor stem cells in serum-free suspension culture of lung cancer cell A549 into single cell suspension. RT-PCR reaction liquid mixed into the chip, and cells were randomly distributed and reverse transcription PCR (RT-PCR)in 2 048 micro chamber.The chip integrated in cell capture,separation,lysing and PCR reaction to achieve rapid identification of tumor stem cells. By counting the number of positive reaction,we could calculate the proportion of tumor stem cell in the total cells.关键词
单细胞/微流控芯片/肿瘤干细胞/分离鉴定/一步法Key words
single cell/microfluidic chip/tumor stem cell/isolation and identification/one step分类
生物科学引用本文复制引用
马丛丛,朱强远,徐亚楠,牟颖..一步法快速分离鉴定肿瘤干细胞的单细胞微流控芯片[J].吉林大学学报（理学版）,2016,54(6):1456-1461,6.基金项目
国家自然科学基金(批准号:31270907)和国家重大仪器设备开发专项基金(批准号:2013YQ470781) （批准号:31270907）
