中国临床保健杂志2016，Vol.19Issue(6)：569-573,5.DOI:10.3969/J.issn.1672-6790.2016.06.003
应用高效液相色谱－二极管阵列检测器筛查中药及保健品中糖皮质激素类非法添加剂
Detection of glucocorticoids illegally adulterated into traditional chinese medicines and health foods by HPLC-DAD
摘要
Abstract
Objective To detect 18 glucocorticoids (hydrocortisone sodium succinate,prednisolone,hydrocorti-sone,prednisone,methylprednisolone,betamethasone,dexamethasone,triamcinolone acetonide,prednisolone acetate,hy-drocortisone,fludrocortisone acetate,prednisone acetate,cortisone acetate,dexamethasone acetate,hydrocortisone buty-rate,triamcinolone acetonide acetate,fluocinonide,halcinonide)illegally adultered into Traditional Chinese Medicines and health foods by establish an HPLC-DAD method.Methods An Alltima C18 column(4.6 mm ×250 mm,5 μm)was used for the separations,The mobile phase were consisted of acetonitrile -10 mmol/L ammonium acetate solution by gra-dient elution.The flow rate was 0.5 mL/min.The column temperature was 30 °C.The detection wavelength was 242 nm, Suspicious samples were confirmed by LC-MS /MS.Results The peaks of hydrocortisone and prednisone were over-lapped,the linearity and sensitivity for other 16 glucocorticoids were fine with all correlation coeffcients (r)bigger than 0.9998 and all LODs between 0.49 ~0.51 ng,The spiked recoveries of low,middle and high levels ranged from 97.5%to 104.2%;the precisions were satisfactory with all RSDs <0.47%;the stability within 12h was acceptable with the RS-Ds of peak areas and retention times no more than 0.68% and 0.35%,Fourteen Traditional Chinese Medicines and health foods were test by HPLC-DAD and LC-MS /MS,and the result showed:dexamethasone acetate was found in a tab-let sample,while betamethasone was found in a spray sample.Conclusion HPLC-DAD is fast and sensitive method for the detection of 18 glucocorticoids illegally adulterated into Traditional Chinese Medicines and health foods.关键词
气相色谱-质谱法/糖皮质激素类/中草药/营养保健品Key words
Mass spectrometry/Glucocorticoids/Drugs,Chinese herbal/Dietary supplements分类
医药卫生引用本文复制引用
徐巧玲,吴学军,姜文清,邝咏梅,金鹏飞,蔡小兵..应用高效液相色谱－二极管阵列检测器筛查中药及保健品中糖皮质激素类非法添加剂[J].中国临床保健杂志,2016,19(6):569-573,5.基金项目
中央保健科研课题专项资金 （）
