药学研究2016，Vol.35Issue(11)：628-631,662,5.DOI:10.13506/j.cnki.jpr.2016.11.002
基于qRT-RNA技术筛选藏药湿生扁蕾抗溃疡性结肠炎肠纤维化活性成分的研究
Bioactive constituents studies on Gentianopsis paludosa against UC fibrosis based on the qRT-RNA method
摘要
Abstract
Objective To study the bioactive constituents of Tibetan medicine Gentianopsis paludosa against UC fibro-sis.Methods Active fragment were obtained by silica gel and TLC from ethyl acetate active part which was extracted from Tibetan Gentianopsis paludosa.By method of qRT-PCR,collagen Ⅰ,collagen Ⅲ,α-SMA,and E-cad were detected to screen the active components after model rats being treated with active isolates.Results After purified and identified from ethyl acetate part using spectroscopy,four monomer compounds(1-hydroxy-3,7,8-Trimethoxyxanthones;1,8-Dihydroxy-3,7-Dimethoxyxanthones;1-hydroxy-3,7-Dimethoxyxanthones and 1,7-Dihydroxy-3,8-Dimethoxyxanthones)had sig-nificant bioactive on UC fibrosis.Conclusion The material basis of Gentianopsis paludosa against fibrosis concentrated in parts of ethyl acetate,and the active ingredient mainly owed to four xanthones.关键词
活性成分/qRT-RNA/湿生扁蕾/溃疡性结肠炎Key words
Active component/qRT-RNA/Gentianopsis paludosa/Ulcerative colitis分类
医药卫生引用本文复制引用
卢年华,赵慧巧,刘雪枫,景明,陈正君..基于qRT-RNA技术筛选藏药湿生扁蕾抗溃疡性结肠炎肠纤维化活性成分的研究[J].药学研究,2016,35(11):628-631,662,5.基金项目
国家自然科学基金项目 （）
