山东医药2016，Vol.56Issue(43)：1-3,3.DOI:10.3969/j.issn.1002-266X.2016.43.001
去水卫矛醇对人脑胶质瘤细胞 BT325增殖、凋亡及线粒体膜电位的影响
Effects of DianhydrogaIactitoI on ceII proIiferation and apoptosis of human gIioma BT325 ceIIs and aIternation in mitochondriaI membrane potentiaI
摘要
Abstract
Objective To investigative the effects of dianhydrogalacitol(DAG)on cell proliferation and apoptosis of human glioma BT325 cells and its potential mechanism.Methods BT325 cells were divided into the control group and DAG-treated groups at various oncentrations.CCK-8 assay and colony formation assay were performed to detect the cell via-bility of BT325 cells.Hoechst33342 was employed to observe nucleus morphological changes and apoptotic rate.The mito-chondrial membrane potential (MMP)was detected by using Rhodamine 123 staining.Results With the increasing DAG concentration,the survival rate of BT325 cells decreased gradually (P <0.05).The survival rate of BT325 cells decreased with the prolonged incubation time in DAG-treated groups (all P <0.05).The IC50 of BT325 cells treated by DAG were 108.81,26.61,and 11.38 μg/mL at 24,48,and 72 h,respectively.The result of colony formation assay showed that DAG inhibited clones of BT325 cells and the colony formation rate in the control group was 16.78% ±1.50% (all P <0.01).The apoptotic rates of DAG-treated groups treated with 5,10,20,40 and 80 μg/mL DAG were 11.79% ± 2.91%,15.42% ±3.06%,38.70% ±1.12%,60.78% ±1.30%,and 80.35% ±3.22%,respectively,which were in an dose-dependent manner and were significantly increased as compared with that (4.27% ±1.72%)of the control group (all P <0.01).The MMP of control group and DAG-treated groups(5,10,20,and 40 μg/mL)were 0.193 ±0.014, 0.174 ±0.038,0.142 ±0.037,0.138 ±0.033,and 0.119 ±0.013,respectively.Significant difference was found in the MMP between the DAG-treated groups (20 and 40 μg/mL)and the control group (P <0.05 and P <0.01).Conclusions DAG inhibits the proliferation and induces apoptosis of BT325 cells.It is suggested that decreased MMP levels might in-volve in the mechanism.关键词
去水卫矛醇/人脑胶质瘤 BT325/细胞增殖/细胞凋亡/线粒体膜电位Key words
Dianhydrogalactitol/human glioma BT325/cell proliferation/apoptosis/mitochondrial membrane potential分类
医药卫生引用本文复制引用
蒋霞,黄银妹,王小洁,刘华钢..去水卫矛醇对人脑胶质瘤细胞 BT325增殖、凋亡及线粒体膜电位的影响[J].山东医药,2016,56(43):1-3,3.基金项目
广西壮族自治区自然科学基金资助项目（2015GXNSFAA139134）。 （）
