山东医药2016，Vol.56Issue(43)：22-25,4.DOI:10.3969/j.issn.1002-266X.2016.43.007
大剂量糖皮质激素应用过程中早期给予 ACTH 大鼠下丘脑－垂体－肾上腺轴的功能及病理变化
FunctionaI and pathoIogicaI changes of HPA axis of rats in the earIy-stage appIication of ACTH during the process of using a Iarge dose of gIucocorticoid
摘要
Abstract
Objective To observe the functional and pathological changes of hypothalamic-pituitary-adrenal (HPA) axis of rats in the early-stage application of adrenocorticotropic hormone (ACTH)during the process of using a large dose of glucocorticoid (GC).Methods Thirty male Wistar rats were randomly divided into the normal group,control group and ACTH treatment group,10 rats in each group.Rats of the normal group were given distilled water by gavage for 2 weeks. Rats of the control group and ACTH treatment group were treated with high dose of prednisone acetate in aqueous solution by gavage for 2 weeks.After that,the ACTH treatment group was treated with subcutaneous injection of ACTH for 2 weeks, and the other two groups were treated with the same treatment as before.The levels of cortisol and ACTH in serum were measured respectively at the beginning of the experiment,at the end of 2 and 4 weeks after the experiment.When the ex-periment was over 4 weeks,the rats were killed,the quality of the pituitary and adrenal glands were measured,the patho-logical changes were observed,and organ index was calculated.Results At the beginning of the experiment,there was no difference in the serum cortisol and ACTH between the groups (P >0.05).At the end of 2 weeks after the experiment, there was no significant difference in serum cortisol and ACTH between the control group and the ACTH treatment group (P>0.05).At the end of 4 weeks after the experiment,the serum cortisol and ACTH levels in the ACTH treatment group were higher than those in the control group (all P <0.05),and the quality of pituitary,adrenal gland and organ index were higher than those of the control group (all P <0.05).At the end of 4 weeks after the experiment,no significant changes were found in the distal part of the pituitary of rats in each group.And the structure of adrenal cortex zona of rats in the nor-mal group was clear.The structure of adrenal cortex zona of the control group was thinning and disordered as compared with that of the normal group.The structure of adrenal cortex zona of the ACTH treatment group was thickening and the nucleus increased at different degrees as compared with that of the control group,but it arranged in disorder as compared with that of the normal group.Conclusion Early application of ACTH in the process of using high dose GC can reduce the inhibito-ry effect of GC on HPA axis in rats,accelerate the adrenal cortex zona secretion,promote the proliferation of pituitary and adrenal gland,and improve the adrenal cortex zona atrophy in pathology significantly.关键词
促肾上腺皮质激素/糖皮质激素/下丘脑 -垂体 -肾上腺轴/原发性肾病综合征/动物实验Key words
adrenocorticotropic hormone/glucocorticoid/hypothalamic-pituitary-adrenal axis/primary nephrotic syn-drom/animal experiment分类
医药卫生引用本文复制引用
杨丽潇,张碧丽,王文红..大剂量糖皮质激素应用过程中早期给予 ACTH 大鼠下丘脑－垂体－肾上腺轴的功能及病理变化[J].山东医药,2016,56(43):22-25,4.基金项目
天津市中医药管理局中医、中西医结合科研专项基金资助项目（13127）。 （）
