山东医药2016，Vol.56Issue(43)：26-29,4.DOI:10.3969/j.issn.1002-266X.2016.43.008
万古霉素依赖性屎肠球菌的生物学特性观察
BioIogicaI characteristics of vancomycin-dependent enterococcus faecium
摘要
Abstract
Objective To observe the biological characteristics of a vancomycin-dependent enterococcus faecium (VDEfm-1501).Methods A strain of VDEfm-1501 was isolated from an urine specimen of a woman with blood system disease and was identified by VITEK 2,followed by PCR and VITEK MS.The VDEfm-1501 vancomycin-dependent growth characteristics and the minimum vancomycin concentration required for growth were tested by E-test.Its antibiotic resist-ance was analyzed by K-B disc diffusion.Drug resistance and virulence genes were amplified by PCR.The multilocus se-quence typing (MLST)was applied in the sequence typing.Ddl sequencing of VDEfm-1501 was detected by PCR.Results VDEfm-1501 could only grow on the presence of 1.0 μg/mL vancomycin at least,but such characteristic could weaken with the increase of passage times,and no vancomycin-dependence was found in the sixth passage.VDEfm-1501 showed multi-drug resistance except linezolid and tetracycline,and carried resistant gene of ace(6')-Ie-aph(2")-la.VDEfm-1501 belonged to clonal complex 17 (CC17),which was the cause of most of the nosocomial VRE outbreaks globally.And its vancomycin dependence seemed to be associated with the insertion of 'GG'at nucleotide position 942 of the ddl gene.Con-clusions The growth of VDEfm-1501 needs at least 1 μg/mL vancomycin,but such dependence is easy to lose.VDEfm-1501 displays multi-drug resistance and carrys the resistant gene of ace(6')-Ie-aph(2")-la.VDEfm-1501 belongs to ST of VRE.The insertion of 'GG'leads to the emergence of VDEfm-1501.关键词
肠球菌/耐万古霉素的肠球菌/万古霉素依赖性肠球菌/屎肠球菌/万古霉素依赖性屎肠球菌/万古霉素/替考拉宁Key words
enterococcus/vancomycin-resistant enterococci/vancomycin-dependent enterococci/enterococcus faeci-um/vancomycin-dependent enterococcus faecium/vancomycin/teicoplanin分类
医药卫生引用本文复制引用
韩清珍,徐杰,张险峰,钱雪峰,章杨,史进方,仇惠英..万古霉素依赖性屎肠球菌的生物学特性观察[J].山东医药,2016,56(43):26-29,4.基金项目
国家自然科学基金青年科学基金资助项目（81501425）；江苏省苏州市“科教兴卫”青年科技项目（kjxw2014008）。 （）
