山东医药2016，Vol.56Issue(43)：34-37,4.DOI:10.3969/j.issn.1002-266X.2016.43.010
早发重度 PE 及 HEELP 综合征患者胎盘组织、母血、脐血中 HMGB1和 RAGP 的表达观察
Expression of HMGB1 and RAGE in pIacenta,maternaI serum and cord serum in patients with earIy-onset severe PE and HELLP syndrome
摘要
Abstract
Objective To observe the expression changes of high mobility group box 1 (HMGB1)and receptor for ad-vanced g1ycation end products (RAGE)in the placenta,maternal serum and cord serum in patients with early-onset severe preeclampsia (PE)and HELLP syndrome,and to analyze the correlation of HMGB1 and RAGE with the pathogenesis of ear-ly-onset severe PE and HELLP syndrome.Methods Thirty patients with early-onset severe preeclampsia (early-onset severe PE group),12 patients with HELLP syndrome (HELLP group)and 30 healthy pregnant women (control group)were recrui-ted in the study.Immunohistochemistry and Western blotting were used to investigate the locations and expression of HMGB1 and RAGE protein in the placentas.Real-time PCR was used to investigate the expression of HMGB1 and RAGE mRNA in the placentas.ELISA was further used to detect the levels of HMGB1 and RAGE protein in maternal and cord serums of the three groups.Results A low level of positive immunostaining for HMGB1 was observed in syncytiotrophoblast cells in pla-centas of control group,the level was significantly increased in syncytiotrophoblast and vascular endothelial cells in placentas of early-onset severe PE group,besides,in the placentas of HELLP group,positive HMGB1 cells were mainly syncytiotropho-blast,macrophage and vascular endothelial cells.A small amount of positive immunostaining for RAGE was observed in syn-cytiotrophoblast and vascular endothelial cells in placentas of the control group,the distribution of positive cells in placentas of the early-onset severe PE group was consistent with that of the control group,what's more,in the placentas of HELLP group,the positive RAGE cells were mainly syncytiotrophoblast and macrophage cells (immunoreactive cells and the expres-sion intensity were significantly higher than those in the early-onset of severe PE group).Compared with the control group, the protein and mRNA levels of HMGB1 and RAGE were increased in the placentas of early-onset PE and HELLP groups, furthermore,the levels of them in HELLP group were even higher than those in the early-onset severe PE group (all P <0.05).In addition,the levels of HMGB1 and RAGE in the maternal serum of early-onset severe PE and HELLP groups were higher than those of the control group,besides,the levels of them in the HELLP group were even higher than those in the ear-ly-onset severe PE group (all P <0.05).However,there were no statistically significant differences in the levels of HMGB1 and RAGE in the cord serum among the three groups (all P >0.05).Conclusion The levels of HMGB1 and RAGE protein increase significantly in the maternal and cord serums of patients with early-onset severe PE and HELLP syndrome,both of which may participate the occurrence and development of early-onset severe PE and HELLP syndrome.关键词
子痫前期/HELLP 综合征/高迁移率族蛋白 1/晚期糖基化终末产物受体Key words
preeclampsia/HELLP syndrome/high mobility group box 1/receptor for advanced glycation end products分类
医药卫生引用本文复制引用
李红,闫欢,朱琳琳,王晓芳,王金铭,冷茂东,张展..早发重度 PE 及 HEELP 综合征患者胎盘组织、母血、脐血中 HMGB1和 RAGP 的表达观察[J].山东医药,2016,56(43):34-37,4.基金项目
河南省高等学校重点研究项目（15A320064）。 （）
