山东医药2016，Vol.56Issue(44)：1-3,3.DOI:10.3969/j.issn.1002-266X.2016.44.001
结肠癌患者血浆外泌体表面膜结合型转化生长因子β1表达及其生物学作用
Expression of mTGF-β1 on the surface of exosomes in plasma from patients with colorectal cancer and its biological function
摘要
Abstract
Objective To explore the membrane-bound transforming growth factor-β1 ( mTGF-β1 ) expression on the surface of exosomes in the plasma from patients with colorectal cancer and its biological function .Methods The expression of mTGF-β1 on the surface of exosomes from 20 samples of patients with colorectal cancer ( observation group ) and 10 healthy donors (control group) were analyzed by flow cytometry.Peripheral blood mononuclear cells (PBMCs) were isolated from heparin anticoagulated whole blood of colon cancer patients , and CD4+T cells were purified and obtained by sorting .Mean-while, CD3 and CD28 were used to induce cell proliferation , and the cells were randomly divided into three groups:groups A, B and C, group A was added with 1μg/ml exosomes from plasma of patients treated with anti-TGF-β1 monoclonal anti-body ( mAb) , group B was added with 1μg/ml exosomes from plasma of patients treated with control IgG 1 mAb and group C was added with 1μg/mL exosomes from plasma of healthy donors .The cell count was calculated by Trypan blue stainin , and the levels of TNF-αand IFN-γwas detected by ELISA on day 3 after culture.Results The positive rate of mTGF-β1 expres-sion in the observation group was 36.52%±4.26%, which was higher than that (9.23%±3.77%) in the control group (P<0.01).Compared with group C, the number of CD4+T cells and the levels of TNF-αand IFN-γsecreted in the group B were all lower than those in the group C (all P<0.05).Compared with group B, the number of CD4+T cells and the levels of TNF-αand IFN-γsecreted in the group A were higher (all P<0.05).Conclusion The mTGF-β1 expression is highly expressed on the surface of exosomes in the plasma from patients with colorectal cancer , and the increased expression of mT-GF-β1 may play an important role in the colon cancer immunosuppressive environment , which promotes the occurrence and development of colon cancer .关键词
结肠癌/膜结合型转化生长因子β1/外泌体Key words
colorectal carcinoma/membrane-bound transforming growth factor-β1/exosomes分类
医药卫生引用本文复制引用
居颂文..结肠癌患者血浆外泌体表面膜结合型转化生长因子β1表达及其生物学作用[J].山东医药,2016,56(44):1-3,3.基金项目
国家自然科学基金资助项目（81373149）；江苏省自然科学基金资助项目（BK20151195）；苏州市科技计划项目（SYS201363）。 （）
