山东医药2016，Vol.56Issue(44)：16-19,4.DOI:10.3969/j.issn.1002-266X.2016.44.005
铜绿假单胞菌临床分离株基因型分析
Genotyping of Pseudomonas aeruginosa clinical isolates
摘要
Abstract
Objective To analyze the genotypes of Pseudomonas aeruginosa ( PA) isolated from inpatients with long-term and continuous infection in various departments of hospital , the and to explore the genetic variation of PA .Methods The strains of PA were collected from specimens of various departments of inpatients , and then we amplified the strains in which the infection lasted for more than 30 days, obtained the RAPD fingerprinting by random amplified polymorphic DNA ( RAPD) genotyping technique , analyzed homology by homology clustering , and understood the genetic relationship be-tween PA through the similarity coefficient .At the same time, we compared the RAPD type to determine whether there was an epidemic outbreak and whether there was a variation between the strains from the same patients with continuous infec -tion.Results A total of 293 strains of PA were collected , including 117 strains in which the infection duration was longer than 30 days, and then 19 genotypes were amplified with A-S type, and the classification rate is 100%.The similarity co-efficient ranged from 0.64 to 0.93, the similarity coefficient of genotypes A and G , genotypes B and R , genotypes D and E, genotypes M and P was 0.93, indicating a high degree of homology between them .In the intensive care unit and burn plastic surgery , the same RAPD type was detected in different patients , the rest of the patients had different RAPD types . The PA fingerprints of 4 patients who suffered continuous infection changed .Conclusion There are many genotypes in PA isolates from patients in various departments of the hospital , which suggests that PA gene mutation occurs in the process of infection, and there is a genetic polymorphism .关键词
铜绿假单胞菌,基因型/随机扩增多态性DNA/基因分型技术Key words
Pseudomonas aeruginosa/random amplified polymorphic DNA/genotypes分类
医药卫生引用本文复制引用
周小青,谢彦彤,梁宏洁,王可,李亚楠,孔晋亮..铜绿假单胞菌临床分离株基因型分析[J].山东医药,2016,56(44):16-19,4.基金项目
国家自然科学基金资助项目（81260663／81460003）；广西自然科学基金资助项目（2013GXNSFAA019166）。 （）
