世界中医药2016，Vol.11Issue(10)：2126-2129,4.DOI:10.3969/j.issn.1673-7202.2016.10.052
ITS2序列鉴定大蓟、小蓟药材及其近缘混伪品
Identification of Cirsium japonicum,Cirsium setosum and Their Adulterants Based on ITS2 Sequence
摘要
Abstract
Objective:To identify C.japonicum, C.setosum and their adulterants through ITS 2 Sequence method to ensure clinical medication safety.Methods:DNA was extracted from C.japonicum and C.setosum, which were the original plant samples of Cir-sii Japonici Herba and Cirsii Herba together with their adulterant and ITS 2 sequences were obtained by PCR , sequencing , and se-quence assembly .Totally, 54 sequences of 14 species were downloaded from the GenBank , using MEGA 6.0 to align all se-quences, calculating the intraspecific and interspecific Kimura-2-Parameter (K2P) genetic distance and constructing NJ tree .Re-sults:It suggested that the intraspecific variation locis between C .japonicum and C .setosum were much less than that between adul-terants.The maximum (average) intraspecific distance of C.japonicum and C.setosum was lower than the minimum (average) interspecific distance of adulterants .Based on ITS2 sequences , this identification method of NJ Tree were effective and clearly dis-tinguished C.japonicum and C.setosum from their adulterant.Also, all species showed obvious monophyly .Conclusion:The se-quence of ITS2 identified the herbal materials of C .japonicum, C.setosum and their Adulterants .关键词
大蓟/小蓟/ITS2序列/鉴定/近缘混伪品Key words
Cirsium japonicum/Cirsium setosum/ITS2 sequence/Identification/Adulterants分类
医药卫生引用本文复制引用
张景景,祁晓婷,张超,朱莉敏,杜康,胡志刚..ITS2序列鉴定大蓟、小蓟药材及其近缘混伪品[J].世界中医药,2016,11(10):2126-2129,4.基金项目
湖北省科技支撑计划(研发与示范类)(编号:2015BCA275):茯苓等8 种湖北优势中药材 DNA 条形码分子鉴定体系构建,负责人:胡志刚 （研发与示范类）
