中国组织工程研究2016，Vol.20Issue(45)：6706-6713,8.DOI:10.3969/j.issn.2095-4344.2016.45.003
非接触共培养条件下骨髓间充质干细胞向类髓核细胞的诱导分化
Effect of indirect co-culture system on differentiation of bone marrow mesenchymal stem cells into nucleus pulposus-like cells
摘要
Abstract
BACKGROUND:Mesenchymal stem cel s (MSCs) for the treatment of degenerative disc diseases present a new therapeutic strategy for the structural and functional recovery of the degenerative intervertebral disc. <br> OBJECTIVE:To study the differentiation potential of rabbit bone marrow MSCs (BMSCs) into nucleus pulposus-like cel s. <br> METHODS:Passage 3 BMSCs and nucleus pulosus cells (NPCs) extracted from rabbits were assigned into simple BMSCs culture, simple NPCs culture or co-culture group. In the former two groups, BMSCs or NPCs were cultured in 6-well culture plates. In the co-culture group, passage 3 BMSCs and NPCs were cultured on the upper or lower layer of the 6-well Transwell chamber, respectively. Cell morphology was observed by inverted contrast phase microscope. At 7 days of culture, immunocytochemistry, RT-PCR and western blot were used to examine the expression levels of type II collagen and aggrecan. <br> RESULTS AND CONCLUSION:At 7 days of co-culture, BMSCs were polygonal or short spindle-shaped, with no fiber-like changes, and cel morphology was close to that of NPCs. Additional y, the expression levels of type II col agen and aggrecan in the BMSCs co-cultured with NPCs were similar to those in the NPCs cultured alone, but significantly higher than those in the BMSCs culture only. Overal , these results show that coculture of BMSCs with NPCs can induce BMSCs differentiating into an NPCs phenotype, indicating that BMSCs may provide sufficient sources of cel s for the biological treatment of degenerative disc diseases.关键词
干细胞/骨髓干细胞/髓核细胞/骨髓间充质干细胞/非接触式共培养/兔/国家自然科学基金分类
医药卫生引用本文复制引用
武海军,银和平,胡继平,刘聪,李树文,白明,杜志才..非接触共培养条件下骨髓间充质干细胞向类髓核细胞的诱导分化[J].中国组织工程研究,2016,20(45):6706-6713,8.基金项目
国家自然科学基金项目(gjzr12287)@@@@Funding:the National Natural Science Foundation of China, No. gjzr12287 （gjzr12287）
