中国组织工程研究2016，Vol.20Issue(45)：6782-6787,6.DOI:10.3969/j.issn.2095-4344.2016.45.014
骨髓间充质干细胞移植治疗骨关节炎
Bone marrow mesenchymal stem cell transplantation for osteoarthritis
吴丽红 1陈涛 2王瑞1
作者信息
- 1. 郑州大学附属郑州中心医院肾病风湿科，河南省郑州市 450007
- 2. 郑州大学附属第一医院风湿免疫科，河南省郑州市 450003
- 折叠
摘要
Abstract
BACKGROUND:As the ability of self-renewal, differentiation and migration into damaged tissues, bone marrow mesenchymal stem cel s have been widely used in a variety of diseases. <br> OBJECTIVE:To explore the therapeutic effect of bone marrow mesenchymal stem cel transplantation on osteoarthritis in rats. <br> METHODS:Thirty-six Wistar rats were randomly assigned into transplantation, model or control group. osteoarthritis models were established in the transplantation and model groups, fol owed by tail vein injection of bone marrow mesenchymal stem cel s (5×107/kg) or the same volume of normal saline, respectively. Rats in the control group were subjected to no treatment. Four weeks after injection, levels of CD4+CD25+regulatory T cel s in the spleen, interleukin-17, tumor necrosis factor-αand transforming growth factor-β1 in serum were detected, and arthritis index and the degree of joint swel ing were evaluated as wel . <br> RESULTS AND CONCLUSION:Compared with the control group, both arthritis index and degree of joint swel ing were increased significantly in the model group (P<0.05), but these indicators exhibited a remarkable improvement after cel transplantation (P<0.05). Levels of CD4+CD25+regulatory T cel s in the spleen in the three groups were ranked as fol ows:the transplantation group>the control group>the model group. The levels of interleukin-17 and tumor necrosis factor-αin serum in the transplantation group were lower than those in the model group but higher than those in the control group (P<0.05). Highest level of transforming growth factor-β1 was obtained in the transplantation group, fol owed by the control group and model group (P<0.05). To conclude, these findings indicate that bone marrow mesenchymal stem cel transplantation exert therapeutic effects in osteoarthritis rats by immune and cytokine regulation.关键词
干细胞/移植/骨髓间充质干细胞/骨关节炎/大鼠/细胞因子/调节性T细胞分类
医药卫生引用本文复制引用
吴丽红,陈涛,王瑞..骨髓间充质干细胞移植治疗骨关节炎[J].中国组织工程研究,2016,20(45):6782-6787,6.
