中国组织工程研究2016，Vol.20Issue(46)：6885-6892,8.DOI:10.3969/j.issn.2095-4344.2016.46.006
佛手柑内酯对双氧水诱导人脐静脉内皮细胞衰老的影响
Effects of Bergapten on the aging of human umbilical vein endothelial cells induced by hydrogen peroxide
摘要
Abstract
BACKGROUND:The effective treatment of traditional Chinese medicine can al eviate the aging of endothelial cel s. Traditional Chinese medicine is difficult to extract, and in contrast, Bergapten is cheap and easily purified, but its antisenescence is little reported. OBJECTIVE:To explore the effect of Bergapten on H2O2-induced senescence of human umbilical vein endothelial cel s. METHODS:Human umbilical vein cel aging model was induced by H2O2, and pretreated with 0.1, 1 and 10μmol/L Bergapten, respectively. Expressions of pRb, pAmpk, pS6, LC3-II, and Beclin-1 protein in each group were detected by western blot assay, and the number of aging cel s and pS6-positive cel s was determined byβ-galactosidase staining and immunofluorescence, respectively. ESULTS AND CONCLUSION:After Bergapten pretreatment, pS6 and pRb protein expressions were significantly downregulated, and the number of cel s positive forβ-galactosidase and pS6 was significantly decreased, while protein expressions of pAmpk, Beclin-1and LC3-II were significantly increased (P<0.05). These findings suggest that Bergapten is able to delay the H2O2-induced aging of human umbilical vein endothelial cel s.关键词
组织构建/血管内皮细胞/佛手柑内酯/pAmpk/mTOR/自噬/衰老/H2O2/β-半乳糖苷酶染色/pS6/Beclin-1/LC3-Ⅱ分类
医药卫生引用本文复制引用
丁燕,傅友,单兰兰,奈文青,吴洪渊,唐亮,陈顺枝,戴萌..佛手柑内酯对双氧水诱导人脐静脉内皮细胞衰老的影响[J].中国组织工程研究,2016,20(46):6885-6892,8.基金项目
广州市科技计划项目(2013J4500040)；广东省产学研项目(2012B091100155)@@@@the Scientific and Technology Planning Project of Guangzhou, No.2013J4500040 （2013J4500040）
the Industry-University-Research Project of Guangdong Province, No.2012B091100155 （）
