中国组织工程研究2016，Vol.20Issue(46)：6915-6921,7.DOI:10.3969/j.issn.2095-4344.2016.46.010
体外构建3D人工皮肤
Artificial skin preparation using three-dimensional printing in vitro
摘要
Abstract
BACKGROUND:Epidermis is unable to differentiate into stratum corneum and other parts in the previous artificial skin constructed using three-dimensional printing. OBJECTIVE:To optimize the method of artificial skin construction to obtain more feasible artificial skin. METHODS:Type I col agen extracted from rat-tail and bovine tail was purified. Primary dermal fibroblasts and keratinocytes were isolated from children’s foreskin, and then embedded in bovine or rat tail col age to construct the dermis;keratinocytes were seeded on the dermis for 7 days, fol owed by 7-day air liquid interface, and the artificial skin was final y achieved. The contraction and hydrophobicity by water contact angle were detected, as wel as the morphology was observed by hematoxylin-eosin staining, sirius red staining and immunofluorescence analysis. RESULTS AND CONCLUSION:Seven days after fibroblasts embedded in col agen, the area of bovine col agen was reduced by 21%, while that of rat tail col agen decreased about a half. The water contact angle of the skin constructed by bovine tail col agen was similar to that of human skin, which was much higher than that of the skin constructed by rat tail col agen. Sirius red staining found that the skin constructed by bovine tail col agen had stronger refractivity, more intact structure and thicker fibers than those of the skin constructed by rat tail col agen. Moreover, more multilayer keratinocytes appeared in the skin constructed by bovine tail col agen through hematoxylin-eosin staining. In conclusion, bovine tail col agen is more available for the artificial skin construction.关键词
组织构建/组织工程/人工皮肤/角质细胞/成纤维细胞/胶原/细胞角蛋白14/饲养层/表皮/国家自然科学基金分类
医药卫生引用本文复制引用
刘钰,沈冲,孟琴..体外构建3D人工皮肤[J].中国组织工程研究,2016,20(46):6915-6921,7.基金项目
国家自然科学基金(21476197)@@@@the National Natural Science Foundation of China, No.21476197 （21476197）
