中国组织工程研究2016，Vol.20Issue(47)：7014-7020,7.DOI:10.3969/j.issn.2095-4344.2016.47.003
新型复合壳聚糖季铵盐纳米粒子抗感染骨水泥的生物学特性
Biological property of a novel bone cement composited by quaternary ammonium salt chitosan nanoparticles
摘要
Abstract
BACKGROUND:Postoperative infection in patients underlying total knee arthroplasty is a long-standing puzzle. Current bone cement with antibiotics cannot effectively treat kidney function deficiency and bacterial resistance. OBJECTIVE:To develop a novel kind of bone cement prepared by quaternary ammonium salt chitosan nanoparticles. METHODS:There were three groups including QCSNP-15, Palacos R and Palacos R+G groups. Morphology of the bone cement in each group was observed using scanning electron microscope, the setting time of bone cement was measured, and the in vitro compression strength, cytotoxicity and antibacterial activity tests were performed. RESULTS AND CONCLUSION:Scanning electron microscope observed that QCSNP-15 was made of quaternary ammonium salt chitosan nanoparticles and methyl methacrylate copolymer, and these nanoparticles distributed onto the material surface. The setting time of QCSNP-15 was longer than that of the Palacos R+G bone cement. The compressive strength of QCSNP-15 was significantly lower than that of the Palacos R bone cement (P<0.05), but was larger than 70 MPa stipulated by ISO 5833. Osteoblasts MC3T3-E1adhered wel on the QCSNP-15, and pseudopodia ful y expanded. Similar findings were observed on the Palacos R+G bone cement. The attachment rate of osteoblasts of the QCSNP-15 at 3 hours was significantly lower than that of the Palacos R+G bone cement (P<0.05). The antibacterial activity did not significantly differ between QCSNP-15 and Palacos R+G bone cements, even after 2-week immersion in the PBS. These findings suggest that QCSNP-15 exhibits appropriate setting time, good biomechanical property and antibacterial activity in vitro with no obvious cytotoxicity.关键词
生物材料/骨生物材料/抗生素骨水泥/全膝关节置换/假体周围感染/肾毒性/壳聚糖/纳米粒子/骨水泥/聚甲基丙烯酸甲酯/抗感染/抗菌活性Key words
Tissue Engineering/Biocompatible Materials/Knee Joint/Chitosan分类
医药卫生引用本文复制引用
贺强,马建兵,孙相祥,赵光辉..新型复合壳聚糖季铵盐纳米粒子抗感染骨水泥的生物学特性[J].中国组织工程研究,2016,20(47):7014-7020,7.基金项目
陕西省科学技术研究发展计划(2013JM4057)；西安市红会医院院级科研基金(YJ2016011)Funding:the Scientific Research Development Plan of Shaanxi Province, No.2013JM4057 （2013JM4057）
the Scientific Research Foundation of Xi’an Honghui Hospital, No. YJ2016011 （）
