中国组织工程研究2016，Vol.20Issue(47)：7027-7033,7.DOI:10.3969/j.issn.2095-4344.2016.47.005
载三联抗痨药硫酸钙/聚氨基酸人工骨植入脊柱结核病灶内：植骨界面的组织学改变
Histological changes of the bone-implant interface after calcium sulfate/polyaminoacid artificial bone carrying triple-anti-tuberculosis drugs is implanted into the spinal tuberculosis focus
摘要
Abstract
BACKGROUND:Most researchers have devoted to study the control-release system in spinal tuberculosis focus and implant material for bone defects, but the effective combination to obtain a novel implant material and its treatment outcomes are rarely reported. OBJECTIVE:To observe the histological change of the implant-bone interface after implantation of the calcium sulfate/polyaminoacid artificial bone carrying triple-anti-tuberculosis drugs including isoniazid, rifampicin and pyrazinamide in a rabbit model of spinal tuberculosis, and to assess the fusion ability of the material. METHODS:Thirty-six New Zealand rabbits were randomly divided into experiment, control and blank control groups (n=12 per group). Models of spinal tuberculosis were established in the rabbits and randomly assigned into either experiment or control groups, fol owed by implantation with the calcium sulfate/polyaminoacid artificial bone carrying triple-anti-tuberculosis drugs including isoniazid, rifampicin and pyrazinamide, or the calcium sulfate/polyaminoacid artificial bone, respectively. Healthy controls received the implantation with calcium sulfate/polyaminoacid artificial bone only. RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that the artificial bone was covered by fibers and tightly adhered to the wound in each group at 2 weeks after implantation, the graft started to degrade obviously at the 4th week, degraded mostly at the 8th week, and disappeared completely at the 16th week. There was obvious cal us formation at 4 weeks after implantation in the experiment group, but the bone healing was later than that of the blank control group. The bone healing ended in the control group. In the blank control group, cal us formation and early osteogenesis appeared at the 4th week, and healed completely at the 16th week. Transmission electron microscope and scanning electron microscope revealed that osteoblasts could crawl through the gap of materials to form new bone. These results suggest that the calcium sulfate/polyaminoacid artificial bone carrying triple-anti-tuberculosis drugs can be used as a scaffold, and osteocytes in the bone defect area can crawl through the gap of materials, thereby promoting bone osseointegration at the bone-implant interface.关键词
生物材料/骨生物材料/骨结核/缓释系统/植入物/骨痂/异烟肼/利福平/吡嗪酰胺/硫酸钙/聚氨基酸人工骨/国家自然科学基金Key words
Tissue Engineering/Tuberculosis, Spinal/Bony Callus分类
医药卫生引用本文复制引用
张卓,孙宇航,耿广起,施建党,王自立,牛宁奎,马文鑫,刘海涛,王骞..载三联抗痨药硫酸钙/聚氨基酸人工骨植入脊柱结核病灶内：植骨界面的组织学改变[J].中国组织工程研究,2016,20(47):7027-7033,7.基金项目
国家自然科学基金(81060149)Funding:the National Natural Science Foundation of China, No.81060149 （81060149）
