中国组织工程研究2016，Vol.20Issue(49)：7327-7333,7.DOI:10.3969/j.issn.2095-4344.2016.49.005
银杏平颤方对帕金森病模型小鼠多巴胺神经元丢失及其凋亡的影响
Effects of Ginkgo biloba Pingchan Recipe on loss and apoptosis of dopamine neurons in mouse models of Parkinson’s disease
吴亚丹 1梁培日 1龙登毅 1高炳淼1
作者信息
- 1. 海南省老年病医院神经内科，海南省海口市 571100
- 折叠
摘要
Abstract
BACKGROUND:Current pharmacological research has administrated that Ginkgo biloba and its extracts can eliminate loss and apoptosis of dopamine neurons by antioxidation and nerve growth factor activation. <br> OBJECTIVE:To confirm the effects of Ginkgo biloba Pingchan Recipe on loss and apoptosis of dopamine neurons as wel as proliferation of neuroblastoma derived cel lines SH-SY5Y in mice with Parkinson’s disease. <br> METHODS:(1) C57BL mice were randomly divided into three groups:20 mice were modeled into Parkinson’s disease by intraperitoneal injection of MPTP for 6 weeks, and at 30 minutes before each intraperitoneal injection, mice in model group were given gavage of normal saline, mice in treatment group given Ginkgo biloba Pingchan Recipe. The other 10 mice received no any interventions as normal group. The loss and apoptosis of dopamine neurons were observed by immunohistochemistry at 15 and 30 days after modeling. (2) In vitro cultured SH-SY5Y cel lines were treated with Ginkgo biloba Pingchan Recipe. Subsequently, the cel proliferation and apoptosis were detected using MTT assay and flow cytometry, respectively;PARP and PTEN mRNA and protein levels were measured through real-time PCR and western blot assay. <br> RESULTS AND CONCLUSION:(1) Compared with the model group, the number of tyrosine hydroxylase-positive neurons at the substantia nigra compacta of midbrain was significantly increased in a time-dependant manner in the normal and treatment groups (P<0.05). (2) The apoptosis rate and mRNA and protein levels of PARP and PTEN in the treatment group were lower than those in the model group. These results suggest that Ginkgo biloba Pingchan Recipe can partly inhibit midbrain neuron apoptosis that may be by decreasing PTEN level. As a result, it becomes possible to prevent and treat Parkinson’s disease via protecting dopamine neurons from loss.关键词
实验动物/神经损伤与修复动物模型/银杏平颤方/帕金森病/小鼠/多巴胺神经元/PTEN/细胞凋亡分类
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吴亚丹,梁培日,龙登毅,高炳淼..银杏平颤方对帕金森病模型小鼠多巴胺神经元丢失及其凋亡的影响[J].中国组织工程研究,2016,20(49):7327-7333,7.
