厦门大学学报（自然科学版）2016，Vol.55Issue(6)：842-846,5.DOI:10.6043/j.issn.0438-0479.201510025
老鹰茶乙醇提取物对α-葡萄糖苷酶的抑制作用
Inhibitory Effect of Ethanol Extract from Hawk Tea onα-Glucosidase
摘要
Abstract
In this study,we explored the inhibitory effect of ethanol extract from Hawk tea onα-glucosidase.We used Hawk tea as the raw material and obtained ethanol extract through ethanol extraction,and then analyzed the main ingredient of the ethanol extract using high performance liquid chromatography.Data showed that the main active substance of ethanol extract from Hawk tea was fla-vonoid constituent,which showed a strong inhibitory effect on the activity ofα-glucosidase.The half maximal inhibitory concentration (IC50 )was 0.12 mg/mL.Furthermore,enzyme kinetics analysis demonstrated a reversible mixed inhibition mechanism,where the inhibition constant for resolvase (KI)was 0.058 mg/mL,while the inhibition constant for enzyme-substrate complexes (KIS)was 0.178 mg/mL.In summary,the ethanol extract from Hawk tea contained bioactive compounds againstα-glucosidase,which could effectively inhibit the activity ofα-glucosidase.This result reveals that Hawk tea is a potential raw material for the development ofα-glucosidase-inhibition relevant health care and medical products.关键词
老鹰茶/α-葡萄糖苷酶/抑制作用/乙醇提取物Key words
Hawk tea/α-glucosidase/inhibition/ethanol extract分类
生物科学引用本文复制引用
芶琳,钟昱阳,阳艺,王西瑶..老鹰茶乙醇提取物对α-葡萄糖苷酶的抑制作用[J].厦门大学学报（自然科学版）,2016,55(6):842-846,5.基金项目
国家自然科学基金(31071433) （31071433）
