医药导报2016，Vol.35Issue(12)：1307-1311,5.DOI:10.3870/j.issn.1004-0781.2016.12.005
参麦注射液诱导人脑胶质瘤SHG-44细胞凋亡的影响∗
Influence of Shenmai Injection on Apoptosis of Human Glioma Cells SHG-44
摘要
Abstract
Objective To investigate growth inhibition and apoptosis induction of Shenmai injection on human glioma cells SHG-44 in vivo and in vitro. Methods Cells were cultivated in vitro, SHG-44 cells in culture medium were treated with 10, 20, 40, 80, 160 and 320 μg·mL-1 Shenmai injection, respectively, the inhibitory rate of the cells was measured by MTT assay after 48, 72 and 96 h respectively. Annexin V-FITC/PI was utilized to measure the apoptosis;SHG-44 cells were seeded in BALB/C-nu mice, the tumor-bearing nude mice were divided into 5 groups randomly: model control group, cisplatin group, Shenmai injection (20, 40, 80 mg·kg-1 ) groups. The mice were intraperitoneally injected daily. General activity and food intake in nude mice were observed, after 12 days. The tumor weight was determined, and the tumor-inhibition rate was calculated. The content of gene protein Caspase-9, Caspase-12, Fas and Survivin were detected by immunohistochemistry. Results Shenmai injection inhibited the proliferation of SHG-44 cells in vitro at six doses, which was concentration- and time-dependent. At a dosage range of 20-80 μmol·L-1 in cultured cells, the apoptosis rates was elevated, Shenmai intraperitoneal injection inhibited the tumor growth of tumor-bearing nude mice, improved the level of Caspase-9, Caspase-12 and Fas, and decreased the level of Survivin. Conclusion Shenmai injection can inhibit proliferation of SHG-44 cells in vitro and in vivo, and induce its apoptosis. The mechanism may be related to the level of Caspase-9, Caspase-12, Fas and Survivin.关键词
参麦注射液/SHG-44细胞/人脑胶质瘤Key words
Shenmai injection/SHG-44 cells/Human glioma cells分类
医药卫生引用本文复制引用
吴炎卿,章激..参麦注射液诱导人脑胶质瘤SHG-44细胞凋亡的影响∗[J].医药导报,2016,35(12):1307-1311,5.基金项目
襄阳市中心医院院级立项(YY20120C04) （YY20120C04）
