中国草食动物科学2016，Vol.36Issue(6)：11-13,3.DOI:10.3969/j.issn.2095-3887.2016.06.003
龙陵黄山羊成纤维细胞系的建立及其生物学特性分析
Establishment and the Biological Characteristics of Fibroblast Cell Line in Yunnan Longling Yellow Goat
摘要
Abstract
In this study,the fibroblasts cell line derived from ear marginal tissue of Longling yellow goat was successfully established using the primary explants technique and cryopreservation technology. The morphology,dynamic growth and kar-yotypes of the cells cultured after different passages were analyzed,the tissue cells of the ear were observed by transmission electron microscopy. The results showed that the population doubling time(PDT)of the cells was 48 h;the growth curve of the cultured cells appeared“S”shape;the frequency of cell chromosome number to be 2n=60 was 91.68%.关键词
龙陵黄山羊/成纤维细胞系/生物学特性Key words
Longling yellow goat/fibroblast cell line/biological characteristics分类
农业科技引用本文复制引用
吕春荣,王思宇,赵其毅,杨维林,邵庆勇,洪琼花..龙陵黄山羊成纤维细胞系的建立及其生物学特性分析[J].中国草食动物科学,2016,36(6):11-13,3.基金项目
云高新产业发展 （）
