中国全科医学2016，Vol.19Issue(36)：4478-4483,6.DOI:10.3969/j.issn.1007-9572.2016.36.015
miR -200 c增加 H2228细胞对克唑替尼和紫杉醇及顺铂敏感性的研究
Effect of Increased H2228 Cells through miR-200c on Sensitivity of Crizotinib, Paclitaxel and Cisplatin
摘要
Abstract
Background The miR-200c can increase the sensitivity of tumor cells on paclitaxel and cisplatin , and the sensitivity of positive lung carcinoma cells in epidermal growth factor receptor ( EGFR) on molecular targeted drug such as erlotinib hydrochloride tablets , gefitinib and afatinib.However, there are no related studies on whether it can increase the sensitivity of echinodermata microtubule -associated protein -like 4 ( EML4 ) -anaplastic lymphoma kinase ( ALK ) fusion gene positive lung adenocarcinoma cells on crizotinib , paclitaxel and cisplatin.Objective To observe whether miR-200c can increase the sensitivity of H 2228 cells on crizotinib , paclitaxel and cisplatin , and to explore its mechanism .Methods From February 2014 to May 2015, H2228 cells were cultured and transfected .According to the different transfectants , the cells were divided into enhanced transfection group ( transfected with miR-200c mimics ), enhanced control group ( transfected <br> miR-200c mimics negative control ), inhibitory transfection group ( transfected with miR-200c inhibitor ) and inhibitory control group (transfected with miR-200c inhibitor negative control ) .The real-time fluorescence quantification PCR method was used to detect the expression level of miR-200c gene in H2228 cells, MTT assay was performed to measure the proliferation level of H2228 cells under antineoplastic drugs 〔ketazidine (50, 100, 200 mmol/L), paclitaxel (6.25, 12.50, 25.00 mmol/L) and cisplatin (25, 50, 100 nmol/L)〕 of different concentrations , Transwell assay was used to measure the migration rate of H2228 cells, Real -time PCR method was applied to detect the expression level of E -cadherin, N -cadherin, corrugated protein and CD 24 mRNA in H2228 cells in transfection group and enhanced control group , Western blotting was used to measure the expression levels of E -cadherin, N -cadherin, corrugated protein and CD 24 in H2228 cells in enhanced transfection group and enhanced control group .Results The gene expression level of miR-200c in H2228 cells in enhanced transfection group was significantly higher than that in the enhanced control group ( P<0.05 ) .The gene expression level of miR-200c in H2228 cells in inhibitory transfection group was significantly lower than that in inhibitory control group (P<0.05).The cell proliferation level of H2228 under the effect of antineoplastic drugs of different concentrations in enhanced transfection group was lower than that in enhanced control group ( P<0.05) .The proliferation level of H2228 cells under the effect of the effect of antineoplastic drugs of different concentrations in inhibitory transfection group was higher than that in inhibitory control group (P<0.05) .The migration rate of H2228 cells in enhanced transfection group was greater than that in enhanced control group (P<0.05) .The migration rate of H2228 cells in inhibitory transfection group was greater than that in the inhibitory control group ( P<0.05 ) .The expression level of E -cadherin and its mRNA in H2228 cells in enhanced transfection group was higher than that in enhancedcontrol group , and the expression level of N -cadherin, corrugated protein , CD24 and their mRNA was lower than that in enhanced control group ( P<0.05 ) .Conclusion Through the mesenchymal epithelial transiton in lung carcinoma cells , miR-200c can enhance the sensitivity of H2228 cells on crizotinib, paclitaxel and cisplatin.关键词
抗药性, 肿瘤/H2228/miR-200c/克唑替尼/紫杉醇/顺铂Key words
Drug resistance, neoplasm/H2228/miR-200c/Crizotinib/Paclitaxel/Cisplatin分类
医药卫生引用本文复制引用
高海祥,曹磊..miR -200 c增加 H2228细胞对克唑替尼和紫杉醇及顺铂敏感性的研究[J].中国全科医学,2016,19(36):4478-4483,6.基金项目
河北省2016年度医学科学研究重点课题计划项目(20160065） （）
