中国比较医学杂志2016，Vol.26Issue(11)：1-5,5.DOI:10.3969/j.issn.1671-7856.2016.11.001
生长激素基因在西藏小型猪不同生长阶段和不同组织器官中的表达
Study on the expression of Growth Hormone gene in different tissues and different growth stages of Tibet-minipigs
摘要
Abstract
Objective To study the expression of Growth Hormone ( GH) gene in different tissues and different growth stages of Tibet-minipigs.Methods The expression level of GH gene in heart, liver, spleen, lung, kidney, muscle, skin tissue of Tibet mini-pig was analyzed by Real-time PCR in 0 years old (1 days), 0.1 years old (36 days), 0.25 years old (90 days), 0.5 years old (180 days), 1 years old (360 days), 2 years old (720 days), and 3 years old (1080 days).Results The expressions of GH mRNA could be detected in various tissues (heart, liver, spleen, lung, kidney, muscle, skin) in different growth stages (0, 0.1, 0.25, 0.5, 1, 2, 3 years old) in Tibet mini-pigs.The GH gene was most highly expressed in skin in 0, 0.5 years old and most lowly expressed in muscle comparing with other tissues.Moreover, the GH gene was also most highly expressed in lung and liver in 0.25, 1 years old and 0.1, 3 years old, respectively .The expression level of GH gene reached the peak in 0.1 years old among different growth stages . Conclusion The expression of GH gene in Tibet mini-pig showed an obvious temporal and spatial specificity .关键词
西藏小型猪/生长激素基因/实时荧光定量PCRKey words
Tibet-minipigs/GH gene/Real-time PCR分类
医药卫生引用本文复制引用
田雨光,王玉珏,岳敏..生长激素基因在西藏小型猪不同生长阶段和不同组织器官中的表达[J].中国比较医学杂志,2016,26(11):1-5,5.基金项目
国家自然科学基金（801402625）；中国博士后自然科学基金（2014M550439）；广东省科技计划项目（2016A030303008）；南方医科大学2015科研助手项目（ C1032246）。 （）
