中国比较医学杂志2016，Vol.26Issue(11)：11-17,7.DOI:10.3969/j.issn.1671-7856.2016.11.003
Bcl－2抑制剂对黄芪注射液减轻缺氧缺糖／复氧复糖大鼠海马神经元凋亡的影响
The influence of Bcl-2 inhibitor on the alleviation action of astragalus injection on apoptosis of hippocamal neurons of rats induced by hypoxia/hypoglycemia and reoxygenation
摘要
Abstract
Objective To observe the influence of Bcl-2 inhibitor (TW-37) on the alleviation action of astragalus injection (AI) on apoptosis of hippocamal neurons of rats induced by hypoxia /hypoglycemia and reoxygenation (H/R). Methods The hippocampal neurons were isolated from fetal rat and cultured for eight days .The cells were randomly divided into six groups:normal control group, H/R group, AI group, AI solution group (sterile deionized water), TW-37 group, TW-37 with AI group.All groups were tested after 0 h, 0.5 h, 2 h, 6 h, 24 h, 72 h and 120 h since they were treated with hypoglycemia and reoxygenation after deprived of oxygen and glucose for 30 min except normal control group . The activity of hippocampal neurons were detected with MTT ,The expression level of Caspase-3 was measured by western blotting, the expression of mRNA of Caspase-3 was tested by RT-PCR.Results Compared with normal control group , aside from 0 h group, the activity of neurons decreased obviously ( P <0.05 ), moreover, the protein and mRNA expressions of Caspase-3 were enhanced significantly (P <0.05) in H/R groups.Compared with H/R group, the activity of neurons increased obviously in astragalus group ( P <0.05 ) , and the expressions of Caspase-3 protein and the expression of Caspase-3 mRNA in the AI groups decreased significantly ( P<0.05) besides 0 h group; but there was no significant difference in both AI solution group、TW-37+H/R and TW-37+AI+H/R group ( P>0.05 ) .Conclusions Astragalus injection to increase the neurons activity and decrease the expression level of Caspase -3 in hippocampal neurons of rat by activating the effect of anti-apoptosis of Bcl-2.Bcl-2 is one of the targets exerting the inhibitory action of astragalus injection on apoptosis of hippocamal neurons of rats induced by hypoxia /hypoglycemia and reoxygenation .关键词
黄芪注射液/Bcl-2抑制剂/缺氧缺糖/复氧复糖/海马神经元/细胞凋亡/Caspase-3Key words
Astragalus injection/Bcl-2 inhibitor/Hypoxia/hypoglycemia and reoxygenation/Hippocamal neurons of rats/Apoptosis/Caspase-3分类
医药卫生引用本文复制引用
董雅洁,高维娟,钱涛,卢锴锋,朱炎杰,谢亚芹..Bcl－2抑制剂对黄芪注射液减轻缺氧缺糖／复氧复糖大鼠海马神经元凋亡的影响[J].中国比较医学杂志,2016,26(11):11-17,7.基金项目
河北省科技支撑计划项目（13272504D）；河北省普通高等学校高层次人才科学研究计划项目（GCC2014031）；河北省教育厅青年基金项目（Q2012002）；承德医学院自然科学研究计划项目（Q201506）；河北省高校重点学科建设项目资助（冀教高【2013】4号病理学与病理生理学）。 （）
