中国比较医学杂志2016，Vol.26Issue(11)：24-30,7.DOI:10.3969/j.issn.1671-7856.2016.11.005
丹酚酸A对心肌缺血再灌注损伤大鼠心率变异性和心肌损伤的影响
Effects of salvianolic acid A on heart rate variability and myocardial injury in myocardial ischemia reperfusion injured rats
摘要
Abstract
Objective To observe the effects of Salvianolic acid A ( SAA) on heart rate variability ( HRV) and myocardial injury in myocardial ischemia reperfusion injured ( MI/RI) rats.Methods Sixty SD rats were divided into 6 groups randomly (n=10);the sham group, the MI/RI model group, high, middle and low-dose SAA group (2 mg/kg, 1 mg/kg and 0.5 mg/kg) and positive control group (2 mg/kg Diltiazem).The MI/RI model was made by ligating the left anterior descending branch of coronary artery in rats .Before ligation, the rats were intravenously administered with corresponding drugs .The changes of electrocardiogram of J point and HRV parameters were analyzed .At the end of reperfusion , the myocardial infarct size was measured by using Evans blue and tetrazolium chloride ( TTC) double staining . The changes of plasma aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase (CK) and its isoenzyme (CK-MB), superoxide dismutase (SOD), malondialdehyde (MDA) and nitric oxide (no) content were also detected.Results Compared with MI/RI model group, SAA low, middle and high dose group and Diltiazem group were significantly reduced the total or average value of J point during reperfusion (P<0.05,P <0.01),reduced myocardial infarct size and inhibited the rising of AST, CK-MB and CK activities(P<0.05,P<0.01), and increased the standard deviation of RR intervals (SDNN), the root-mean-square of successive differences (RMSSD), HRV triangle index (tr. Ind) and high frequency (HFnu), reduced the low frequency (LFnu) and the ratio of LF/HF(P<0.05, P<0.01). Moreover , SAA could significantly increase the content of NO and reduce the content of MDA ( P<0.05 ) .Conclusion SAA could increase HRV and reduce myocardial infarction area in MI /RI rats, its mechanism of action maybe related with antioxidant effects .关键词
丹酚酸A/心肌缺血再灌注损伤/心率变异性/抗氧化/大鼠Key words
Salvianolic acid A/Myocardial ischemia reperfusion injury/Heart rate variability/Anti-oxidation/Rats分类
医药卫生引用本文复制引用
王木兰,潘永明,金敏,徐孝平,王德军,马全鑫,陈民利..丹酚酸A对心肌缺血再灌注损伤大鼠心率变异性和心肌损伤的影响[J].中国比较医学杂志,2016,26(11):24-30,7.基金项目
浙江省中医药管理局重点项目（2015ZZ009）；浙江中医药大学比较医学创新团队（ XTD201301）。 （）
